Increase in Bcl2 expression of penile and prostate cells of Sprague Dawley male rats following treatment with buceng (combination of Pimpinella alpina molk with Eurycoma longifolia Jack)
Abstract
Background: Treatment with buceng combination of Eurycoma longifolia Jack and Pimpinella alpine Molk has been proven to increase testosterone level, decrease apoptosis and caspase3 expression. Bcl2 is an antiapoptotic protein found in cytoplasm which inhibits cells apoptosis. This study was aimed to investigate the effect of buceng on Bcl2 expression on penile and prostate tissues of the rats.
Methods: In this experimental study, 24 male Sprague Dawley rats of 90 days old, weighing ± 300 grams, were randomly assigned into four groups. Group A, normal rats. Group B, castrated rats and treated with buceng 100 mg/day, per oral (Cast-Bcg); Group C, castrated rats and treated with 2 ml of water as placebo against buceng (Cast-Plac). Group D, castrated rats, treated with mesterolone 6.75 mg/day, per oral, as exogenous testosterone (Cast-Mest). All rats were treated for 30 days. Manova test was used to analyze the different expression of Bcl2 among groups with significance level at p ≤ 0.05.
Results: Castration was associated with significant decrease of Bcl2 expression in the penile and prostate tissues (53.0 and 50.9%, respectively) compared to normal rats (82.6 and 84.2%, respectively, p < 0.001). Treatment with mesterolone reversed Bcl2 expression (77.1 and 78.1%) to a near normal level. The same level of Bcl2 expression was also observed with buceng treatment (73.8 and 78.2%).
Conclusion: The treatment with buceng could enhance Bcl2 expression in penile and prostate tissues, comparable to normal rats and mesterolone treated rats.
References
Nainggolan O, Simanjuntak JW. Pengaruh ekstrak etanol akar pasak bumi (Eurycoma longifolia Jack) terhadap perilaku seksual mencit putih [the effect of Eurycoma longifolia Jack ethanol extract on sexual behaviour in mice]. Cermin D Kedokteran. 2005;146:55-7. Indonesian.
Darwati I, Roostika I. The Pimpinella alpina Molk research status in Indonesia. Buletin P Nutfah. 2006;12(1):9-15. Indonesian.
Taufiqurrachman. The effect of buceng extracts on androgen production in Sprague Dawley male rats. Med J Indones. 2012;21(1):28-31. http://dx.doi.org/10.13181/mji.v21i1.473
Taufiqurrachman. Extract of Pimpinella alpina Molk and Eurycoma longifolia Jack increase testosterone level, decrease apoptosis in penis and prostate cells. Med M Indonesiana. 2012;46:34-40.
Taufiqurrachman. Decreased expression of caspase3 in penis and prostate tissues of rat after treatment with buceng (Pimpinella alpina Molk) & Eurycoma longifolia Jack). Med J Indones. 2013;22:2-8. http://dx.doi.org/10.13181/mji.v22i1.512
Godfrey B, Lin Y, Larson J, Haferkamp B, Xiang J. Proteasomal degradation unleashes the pro-death activity of androgen receptor. Cell Res. 2010;20(10):1138-47. http://dx.doi.org/10.1038/cr.2010.65
Frezza M, Yang H, Dou QP. Modulation of the tumor cell death pathway by androgen receptor in response to cytotoxic stimuli. J Cell Physiol. 2011;226(11):2731-9. http://dx.doi.org/10.1002/jcp.22758
Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 2008;9(1):47-59. http://dx.doi.org/10.1038/nrm2308
Bogner C, Leber B, Andrews DW. Apoptosis: embedded in membranes. Curr Opin Cell Biol. 2010;22(6):845-51. http://dx.doi.org/10.1016/j.ceb.2010.08.002
Gump MJ, Thorburn A. Autophagy and apoptosis: what is the connection?. Trends Cell Biol. 2011;21(7):387-92. http://dx.doi.org/10.1016/j.tcb.2011.03.007
Sharifi AM, Mottaghi S. Finasteride as a potential tool to improve mesenchymal stem cell transplantation for myocardial infarction. Med Hypotheses. 2012;78(4):465-7. http://dx.doi.org/10.1016/j.mehy.2011.12.021
Libera DL, Ravara B, Gobbo V, Barbera MD, Angelini A, Vescovo G. Therapeutical treatments for blocking apoptosis and preventing skeletal muscle myopathy in heart failure. Basic Appl Myol. 2002;12(2):65-71.
Danial NN, Korsmeyer SJ. Cell death: critical control points. Cell. 2004;116(2):205-19. http://dx.doi.org/10.1016/S0092-8674(04)00046-7
Arnoult D. Mitochondrial fragmentation in Apoptosis. TRENDS in Cell Biology. 2006;17(1):6-12. http://dx.doi.org/10.1016/j.tcb.2006.11.001
Valdivia I, Campodónico I, Tapia A, Capetillo M, Espinoza A, Lavín P. Effects of tibolone and continuous combined hormone therapy on mammographic breast density and breast histochemical markers in postmenopausal women. Fertil Steril. 2004;81(3):618-23. http://dx.doi.org/10.1016/j.fertnstert.2003.07.041
Nieschlag E, Behre HM, Bouchard P, et al. Testosterone replacement therapy: current trends and future directions. Hum Reprod Update. 2004;10(5):409-19. http://dx.doi.org/10.1093/humupd/dmh035
Barceloux DG, Palmer RB. Anabolic-androgenic steroids. Dis Mon. 2013;59(6):226-48. http://dx.doi.org/10.1016/j.disamonth.2013.03.010
Shittu LAJ, Shittu RK, Osinubi AA, Ashiru OA. Stereological evidences of epithelial hypoplasia of seminiferous tubules induced by mesterolone in adult Sprague-Dawley rats. Afr J Endoc and Met. 2008;7:14-7.
Gauthaman K, Adaikan PG, Prasad RN. Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats. Life Sci. 2002;71(12):1385-96. http://dx.doi.org/10.1016/S0024-3205(02)01858-1
Gauthaman K, Adaikan PG, Prasad RNV. Sexual effects of puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation using a rat model. J Altern Complement Med. 2003;(9)2:257-65. http://dx.doi.org/10.1089/10755530360623374
Meny S. Efek ekstrak purwoceng (Pimpinella alpina Molk) terhadap peningkatan biomarker fungsi ereksi pada tikus jantan Sprague-Dawley [dissertation]. Semarang: Diponegoro University; 2013. Indonesian.
Gauthaman K, Ganesan AP. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction--an evaluation using primates, rabbit and rat. Phytomedicine. 2008;15(1-2):44-54. http://dx.doi.org/10.1016/j.phymed.2007.11.011
Viktorov I, Bozadjieva E, Protich M, et al. Pharmacological, pharmacokinetic, toxicological and clinical studies on protodioscin. IIMS Therapeutic Focus. 1994. Available from: http://www.libilov.com/en/clinical_studies/study_IIMS_1994.htm
De Almeida FF, Andersson E, Mittelholzer C, Karlsen O, Taranger GL, Schulz RW. Pituitary gonadotropin and testicular gonadotropin receptor expression in Atlantic cod (Gadusmorhua L.) during the first reproductive season: Effects of photoperiod modulation. Gen Comp Endocrinol. 2011;173(1):111-9. http://dx.doi.org/10.1016/j.ygcen.2011.05.002
Kim HH, Schlgel PN. Endocrine manipulation in male infertility. Urol Clin North Am. 2008;35(2):303-18. http://dx.doi.org/10.1016/j.ucl.2008.01.003
Manitto P. Biosynthesis of natural product. Connecticut. New Delhi: India; 1981. p. 236.
Morley JE, Malmstrom TK. Frailty, sarcopenia, and hormones. Endocrinol Metab Clin North Am. 2013;42(2):391-405. http://dx.doi.org/10.1016/j.ecl.2013.02.006
Ohta Y, Nishikawa A, Fukazawa Y, Urushitani H, Matsuzawa A, Nishina Y, et al. Apoptosis in adult mouse testis induced by experimental cryptorchidism. Acta Anat (Basel). 1996;157(3):195-204. http://dx.doi.org/10.1159/000147881
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