Permanent flame-blunted monofilament of middle cerebral artery occlusion technique for ischemia stroke induction in animal models

  • Yetty Ramli Department of Neurology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta
  • Ahmad S. Alwahdy Department of Neurology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta
  • Mohammad Kurniawan Department of Neurology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta
  • Berry Juliandi Department of Biology, Institut Pertanian Bogor, Bogor
  • Puspita E. Wuyung Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta
  • Yayi D.B. Susanto Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta
DOI: https://doi.org/10.13181/mji.v26i3.1645
Keywords: middle cerebral artery occlusion, monofilament, rat
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Abstract

Background: Rat is the most frequently used animal for ischemic stroke studies. Recently, middle cerebral artery occlusion (MCAO) by introducing various types of surgical monofilament intraluminally has been widely used, with their advantages and disadvantages. For permanent occlusion, problems with mortality in rats are higher than transient. In this study, we used permanent occlusion using modified monofilament by flaming on its tip which may reduce mortality rate, so that chronic phase of stroke can be learned extensively.

Methods: Three male Sprague-Dawley rats underwent permanent MCAO. The flame-blunted monofilament was introduced through common carotid artery. Hematoxylin eosin histopathology confirmation and functional assessment post-stroke induction were then evaluated.

Results: Evaluation was conducted on 3 rats in different time post-stroke induction (48 hours, 72 hours, and 3 weeks). Using histopathological examination, the infarction was proved in all 3 rats showing red neurons, perivascular edema and neutrophil spongiosis, in infarct and peri-infarct area. The changes in histopathology showed spongiosis were more dominant in 3 week-post-MCAO rats. On the other hand, red neurons and perivascular edema were less compared to 48 and 72-hour-post-MCAO rats.

Conclusion: Flame-blunted monofilament showed its efficacy in producing infarct area. The advantages of this technique are easy to perform with simple and less expensive modification of the monofilament. Conducting successful permanent occlusion with less mortality rate will give chances to do further research on stroke in chronic phase and its effect on novel treatment.

References

  1. Bacigaluppi M, Comi G, Hermann DM. Animal models of ischemic stroke. Part two; modelling cerebral ischemia. Open Neurol J. 2010;4:34-8. https://doi.org/10.2174/1874205X01004010034

  2. Park SY, Marasini S, Kim GH, Ku T, Choi C, Park MY, et al. A method for generate a mouse model of stroke: evaluation of parameters for blood flow, behavior, and survival. Exp Neurobiol. 2014;23(1):104-14. https://doi.org/10.5607/en.2014.23.1.104

  3. Kaya AH, Erdogan H, Tasdemiroglu E. Searching evidences of stroke in animal models: a review of discrepancies. Turk Neurosurg. 2016;27(2):167-73. https://doi.org/10.5137/1019-5149.JTN.15373-15.2

  4. Casals JB, Pieri NCG, Feitosa MLT, Ercolin ACM, Roballo KCS, Barreto RSN, et al. The use of animal models for stroke research: a review. Comp Med. 2011;61(4):305-13.

  5. Molina CA, Saver JL. Extending reperfusion therapy for acute Ischemic stroke. Emerging pharmacological, mechanical, and imaging stategies. Stroke. 2005;36:2311-20.

  6. Saver JL, Goyal M, vander Lugt A, Menon BK, Majole CBLM, Dippel DW, et al. Time to treatment with endovascular thrombectomy and outcomes from Ischemic stroke: a meta-analysis. JAMA. 2016;316:1279-88. https://doi.org/10.1001/jama.2016.13647

  7. Fluri F, Schuhmann MK, Kleinschnitz C. Animal models of Ischemic stroke and their application in clinical research. Drug Des Devel Ther. 2015;9:3445-54. https://doi.org/10.2147/DDDT.S56071

  8. Canazza A, Minati L, Boffano C, Parati E, Binks S. Experimental models of brain ischemia: a review of techniques, magnetic resonance imaging, and investigational cell-based therapies. Front Neurol. 2014;5:1-9. https://doi.org/10.3389/fneur.2014.00019

  9. Longa, EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 1989;20:84-91. https://doi.org/10.1161/01.STR.20.1.84

  10. Chou WH., Choi DS, Zhang H, Mu D, McMahon T, Kharazia VN, et al. Neutrophil protein kinase Cdelta as a mediator of stroke-reperfusion injury. J Clin Invest. 2004;114:49-56. https://doi.org/10.1172/JCI200421655

  11. Macrae IM. Preclinical stroke research-advantages and disadvantages of the most common rodent models of focal ischaemia. Br J Clin Pharmacol. 2011;164:1062-78. https://doi.org/10.1111/j.1476-5381.2011.01398.x

  12. Rupadevi M, Parasuraman S, Raveedran R. Protocol for middle cerebral artery occlusion by an intraluminal suture method. J Pharmacother. 2011;2:36â9. https://doi.org/10.4103/0976-500X.77113

  13. Guan Y,Wang Y, Yuan F, Lu H, Ren Y, et al. Effect of Suture Properties on Stability of Middle Cerebral Artery Occlusion Evaluated by Synchrotron Radiation Angiography. Stroke. 2012;43:888-91. https://doi.org/10.1161/STROKEAHA.111.636456

  14. Lubjuhn J, Gastens A, Von WG, Bargiotas P, Herrmann O, Murikinati S, et al. Functional testing in a mouse stroke model induced by occlusion of the distal middle cerebral artery. J Neurosci Methods. 2009;184:95-103. https://doi.org/10.1016/j.jneumeth.2009.07.029

  15. Rosell A, Agin V, Rahman M, Morancho A, Ali C, Koistinaho J, et al. Distal occlusion of the middle cerebral artery in mice: Are we ready to assess long-term functional outcome? Transl Stroke Res. 2013;4:297-307. https://doi.org/10.1007/s12975-012-0234-1

  16. Margatitescu O, Mogoanta L, Pirici I, Pirici D, Cernea D,l. Histopathological changes in acute ischemic stroke. Rom J Morphol Embryol. 2009;50:327-39.

  17. Colak G, Fillano A J, Johnson GV. The application of permanent middle cerebral artery in the mouse. J Vis Exp. 2011;53:1-4. https://doi.org/10.3791/3039

  18. Tamura A, Graham DI, McCulloch J, Teasdale GM . Focal cerebral ischemia in the rat: 1. Description of technique and early neuropathological consequences following middle cerebral artery occlusion. J Cereb Blood Flow Metab. 1981;1:53-60. https://doi.org/10.1038/jcbfm.1981.6

  19. Van Bruggen N, Thibodeaux H, Palmer JT, Lee WP, Fu L, Cairns B et al. VEGF antagonism reduces edema formation and tissue damage after ischemia/reperfusion injury in the mouse brain. J Clin Invest. 1999;104:1613â20. https://doi.org/10.1172/JCI8218

  20. Kano T, Katayama Y, Tejima E, Lo EH. Hemorrhagic transformation after fibrinolytic therapy with tissue plasminogen activator in a rat thromboembolic model of stroke. Brain Res. 2000;854:245-8. https://doi.org/10.1016/S0006-8993(99)02276-3

  21. Li F, Omae F, Fisher M. Spontaneous hyperthermia and its mechanism in the intraluminal suture middle cerebral artery occlusion model of rats. Stroke. 1999;30:2464-70. https://doi.org/10.1161/01.STR.30.11.2464

  22. Herson PS., Traystman R. Animal model of stroke: translational potential at present and in 2050. Future Neurol. 2014;9:541-51. https://doi.org/10.2217/fnl.14.44

  23. Braeuninger S, Kleinschnitz C. Rodent models of focal cerebral ischemia: procedural pitfalls and translational problems. Exp Transl Stroke Med. 2009;1:8. https://doi.org/10.1186/2040-7378-1-8

  24. Guzel A, Rolz R, Nikkhah G, Kalert U.D, Maciaczyk J. A microsurgical procedure for middle cerebral artery occlusion by intraluminal monofilament insertion technique in the rat: a special emphasis on the methodology. Exp Transl Stroke Med. 2014;6:6. https://doi.org/10.1186/2040-7378-6-6

  25. Yi Yang, Gary A.R. Blood-brain barrier breakdown in acute and chronic cerebrovascular disease. Stroke. 2011;42:3323-8. https://doi.org/10.1161/STROKEAHA.110.608257

Published
2017-11-27
How to Cite
1.
Ramli Y, Alwahdy AS, Kurniawan M, Juliandi B, Wuyung PE, Susanto YD. Permanent flame-blunted monofilament of middle cerebral artery occlusion technique for ischemia stroke induction in animal models. Med J Indones [Internet]. 2017Nov.27 [cited 2024Apr.20];26(3):183-9. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1645
Issue
Vol. 26 No. 3 (2017): September
Section
Basic Medical Research

Copyright (c) 2017 Yetty Ramli, Ahmad S. Alwahdy, Mohammad Kurniawan, Berry Juliandi, Puspita E. Wuyung, Yayi D.B. Susanto

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