The profile of codon 200 β-tubulin gene of Ascaris lumbricoides L. and Trichuris trichiura L. from infected people in Nangapanda Sub-district, East Nusa Tenggara
Abstract
Background: The mass administration of anthelmintic such as albendazole is one of the strategies for eliminating soil-transmitted helminth (STH) infection. The widespread and long-term use of anthelmintics can cause resistance. The research on animals shows that factor that the single-nucleotide polymorphism (SNP) codon 200 β-tubulin gene of the worms is one of the factors that can cause the decreased efficacy of anthelmintics. This study aimed to determine the bases of codon 200 in A. lumbricoides and T. trichiura, which infect the people in Nangapanda, East Nusa Tenggara.
Methods: The worm samples were obtained from the intestinal helminth-infected patients from Nangapanda Sub-district. The DNA from the worm tissues were isolated, amplificated by polymerase chain reaction (PCR), and sequenced. The sequencing results were aligned to the reference sequence to obtain the codon bases in the 200 β-tubulin gene.
Results: TTC constitute the codon bases in the 200 β-tubulin gene found in two A. lumbricoides and one T. trichiura.
Conclusion: The SNP codon 200 β-tubulin gene was absent in A. lumbricoides or T. trichiura worms that were examined in this study.
References
Diawara A, Halpenny CM, Churcher TS, Mwandawiro C, Kihara J, Kaplan RM, et al. Association between response to albendazole treatment and β-tubulin genotype frequencies in soil-transmitted helminths. PLOS Negl Trop Dis. 2013;7(5):e2247. https://doi.org/10.1371/journal.pntd.0002247
Vercruysse J, Albonico M, Behnke JM, Kotze AC, Prichard RK, McCarthy JS, et al. Is anthelmintic resistance a concern for the control of human soil-transmitted helminths? Int J Parasitol Drugs Drug Resist. 2011;1(1):14–27. https://doi.org/10.1016/j.ijpddr.2011.09.002
Diawara A, Drake LJ, Suswillo RR, Kihara J, Bundy DA, Scott ME, et al. Assay to detect β-tubulin codon 200 polymorphism in Trichuris trichiura and Ascaris lumbricoides. PLOS Negl Trop Dis. 2009;3(3):e397. https://doi.org/10.1371/journal.pntd.0000397
Hotez PJ, Bundy DAP, Beegle K, Brooker S, Drake L, Silva ND, et al. Chapter 24: Helminth infections: soil-transmitted helminth infections and schistosomiasis. Disease control priorities in developing countries (second ed). Washington (DC): World Bank; 2006. 467–83.
World Health Organization. Prevention and control of schistosomiasis and soil-transmitted helminthiasis. WHO Technical Report Series Report 912. Geneva: WHO; 2002. 33–5.
Uneke CJ. Soil transmitted helminth infections and schistosomiasis in school age children in sub-Saharan Africa: efficacy of chemotherapeutic intervention since World Health Assembly Resolution 2001. Tanzan J Health Res. 2010;12(1):86–99. https://doi.org/10.4314/thrb.v12i1.56366
Keiser J, Utzinger J. Efficacy of current drugs against soil-transmitted helminth infections systematic review and meta-analysis. JAMA. 2008;299(16):1937–48. https://doi.org/10.1001/jama.299.16.1937
Prichard RK. Markers for benzimidazole resistance in human parasitic nematodes? Parasitology 2007;134(8):1087–92. https://doi.org/10.1017/S003118200700008X
Horton J. Albendazole: a review of anthelmintic efficacy and safety in humans. Parasitology. 2000;121:S113–32. https://doi.org/10.1017/S0031182000007290
Wecker L, Crespo LM, Dunaway G, Faingold C, Watts S. Chapter 52: Drugs to treat parasitic infection. Brody's human pharmacology molecular to clinical (fifth ed). Philadelphia, PA (US): Mosby and Elsevier. 2010. 621–30.
Campbell NA, et al. Sitoskeleton adalah jejaring serat yang mengorganisasi struktur dan aktivitas dalam sel. Biologi (edisi delapan). Jakarta: Erlangga; 2010. 120–6.
Kukuljan I. Microtubules: from classical properties to quantum effects in human cognition. Seminar class at the Faculty for Mathematics and Physics of University of Ljubljana. 2013:1–15.
Haryuningtyas D, Beriajaya, Gray GD. Anthelmintic resistance against benzimidazole group on sheep and goat. Seminar nasional teknologi peternakan dan veteriner. 2001: 509–18.
Haryuningtyas D. Deteksi mutasi pada gen tubulin β isotipe-1 cacing Haemonchus contortus isolat resisten terhadap benzimidazole dengan single strand conformation polymorphism. Jurnal Ilmu Ternak dan Veteriner (JITV). 2005;10(3):200–7.
Kwa MS, Veenstra JG, Roos MH. Benzimidazole resistance in Haemonchus contortus is correlated with a conserved mutation at amino acid 200 in β-tubulin isotype 1. Mol Biochem Parasitolol. 1994;63(2):299–303. https://doi.org/10.1016/0166-6851(94)90066-3
Wiria AE, Hamid F, Wammes LJ, Kaisar MM, May L, Prasetyani MA, et al. The effect of three-monthly albendazole treatment on malarial parasitemia and allergy: a household-based cluster-randomized, double-blind, placebo-controlled trial. PLOS One. 2013;8(3):e57899. https://doi.org/10.1371/journal.pone.0057899
DNeasy® blood and tissue handbook. [internet] 2016 [diunduh pada 2016 01 20]. Available at: http://diagnostics1.com/MANUAL/General_Qiagen.pdf.
Hansen TV, Thamsborg SM, Olsen A, Prichard RK, Nejsum P. Genetic variations in the beta-tubulin gene and the internal transcribed spacer 2 region of Trichuris species from man and baboons. Parasit Vectors. 2013;6:236. https://doi.org/10.1186/1756-3305-6-236
DNA protocols and applications. [internet] 2016 [downloaded on 2016 01 20]. Available: https://www.qiagen.com/de/resources/molecular-biology-methods/dna/#Sample storage prior to extraction of genomic DNA.
260/280 and 260/230 ratios. [internet] 2016 [downloaded on 2016 06 09]. Available at: http://www.nanodrop.com/Library/T009-NanoDrop%201000-&-NanoDrop%208000-Nucleic-Acid-Purity-Ratios.pdf.
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