Domain 15 of the serine proteinase inhibitor LEKTI blocks HIV infection in vitro

  • David Palesch Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany
  • Edina Varga Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany
  • Ute C. Marx Lehrstuhl Biopolymere, University of Bayreuth, Bayreuth, Germany
  • Klaus Vitzithum Lehrstuhl Biopolymere, University of Bayreuth, Bayreuth, Germany
  • Peter Kreutzmann IPF PharmaCeuticals GmbH, Hanover, Germany
  • Wolf-Georg Forssmann Clinic of Immunology and Rheumatology, Peptide Research Group, Hanover Medical School, Hanover, Germany
  • Jan Münch Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany
  • Hans-Jürgen Mägert Institute of Biotechnology, Anhalt University of Applied Sciences, Köthen, Germany
DOI: https://doi.org/10.13181/mji.v22i3.580
Keywords: HIV, inhibition, LEKTI, P4-R5 MAGI
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Abstract

Background: Lympho-epithelial Kazal-type-related inhibitor (LEKTI) is a 15-domain serine proteinase inhibitor, parts of which have first been isolated from human blood filtrate. It is encoded by the gene SPINK5. In the past, different groups reported antiviral activities of certain serine proteinase inhibitors, such as mucous proteinase inhibitor and alpha1-proteinase inhibitor. The purpose of this study was to test two representative domains of the proteinase inhibitor LEKTI for anti-HIV activities.

Methods: LEKTI domains 6 and 15 were recombinantly produced in E.coli. To test their inhibitory activity against HIV infection, the reporter cell line P4-R5 MAGI carrying an HIV-inducible reporter gene was infected by a CCR5-tropic HIV strain in the presence of different inhibitor concentrations. After three days, infection rates were determined by quantifying ß-galactosidase activities using the Galacto-Light Plus™ ß-Galactosidase Reporter Gene Assay.

Results: In contrast to LEKTI domain 6, LEKTI domain 15 suppressed HIV-induced reporter gene activities with an IC50 value of approximately 29 μM.

Conclusion: LEKTI domain 15 represents an inhibitor of HIV infection.

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Published
2013-08-22
How to Cite
1.
Palesch D, Varga E, Marx UC, Vitzithum K, Kreutzmann P, Forssmann W-G, MünchJ, MägertH-J. Domain 15 of the serine proteinase inhibitor LEKTI blocks HIV infection <em>in vitro</em&gt;. Med J Indones [Internet]. 2013Aug.22 [cited 2024Apr.23];22(3):131-5. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/580
Issue
Vol. 22 No. 3 (2013): August
Section
Basic Medical Research

Copyright (c) 2013 David Palesch, Edina Varga, Ute C. Marx, Klaus Vitzithum, Peter Kreutzmann, Wolf-Georg Forssmann, Jan Münch, Hans-Jürgen Mägert

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