@article{Handono_Dantara_Dewi_Pratama_Nurdiana_2018, title={<em>Bryophyllum pinnatum</em> leaves ethanol extract inhibit maturation and promote apoptosis of systemic lupus erythematosus BALB/c mice B cells}, volume={26}, url={http://mji.ui.ac.id/journal/index.php/mji/article/view/1899}, DOI={10.13181/mji.v26i4.1899}, abstractNote={<p><strong>Background:</strong> B cells play a key role in systemic lupus erythematosus (SLE). Targeting B cells as SLE therapy is a plausible approach. This study investigated the potential effects of <em>Bryophyllum pinnatum</em> leaves with ethanol extract in decreasing percentages of maturation, increasing percentages of apoptosis, and decreasing&nbsp;NF-κB p65 expressions of SLE BALB/c mice B cells.</p> <p><strong>Methods:</strong> Culturing B cells from pristane induced SLE BALB/c mice’s spleen will resulted in this in vitro study. B cells were activated by BAFF, LPS, IL-4, and anti-CD40 yielding CD19+ &gt;80%. B cells were cultured by adding those stimulants with and without <em>B. pinnatum</em> leaves (0, 0.02, 0.1, or 0.5 µg/ml) for 72 hours at 37°C. Flow cytometry was performed to determine The Percentages of maturation (CD19+CD38+) and apoptosis (Annexin V+PI+) of B cells. Further analysis to determine the expressions of transcription factor of maturation and apoptosis of B cells, NF-κB p65, were performed using immunocytochemistry. Data were analyzed using SPSS version 22.</p> <p><strong>Results:</strong> Flow cytometry assay showed significant decrease in percentages of maturation of B cells in all doses and significant increase in percentage of apoptosis of B cells in dose 0.5 µg/ml. Immunocytochemistry results showed significant decrease expressions of NF-κB p65 in all doses. Percentages of maturation, apoptosis, and expressions of NF-κB p65 of B cells were significantly correlated.</p> <p><strong>Conclusion:</strong> This in vitro study revealed that <em>B. pinnatum</em> leaves with ethanol extract decreased the percentages of maturation, increased the percentage of apoptosis, and decreased NF-κB p65 expressions of SLE BALB/c mice B cells significantly.</p&gt;}, number={4}, journal={Medical Journal of Indonesia}, author={Handono, Kusworini and Dantara, Tri W.I. and Dewi, Elvira S. and Pratama, Mirza Z. and Nurdiana, Nurdiana}, year={2018}, month={Feb.}, pages={253-60} }