@article{Rahayu_Gondhowiardjo_Artini_Wanandi_2020, title={Tear hypoxia-inducible factor-1α expression, lactate dehydrogenase, and malate dehydrogenase activity changes in soft contact lens wear}, volume={29}, url={http://mji.ui.ac.id/journal/index.php/mji/article/view/3405}, DOI={10.13181/mji.oa.203405}, abstractNote={<p><strong>BACKGROUND</strong> Soft contact lens (SCL) wear can lead to a corneal hypoxia. However, there is a lack of studies looking for corneal hypoxia biomarkers in tear. This study aimed to investigate corneal hypoxia among SCL wearers based on hypoxia-inducible factor-1α (HIF-1α) expression, tear lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) activities.</p> <p><strong>METHODS</strong> A nonrandomized clinical trial was conducted on two groups. SCLs were prescribed for 2 months to a group of new wearers. Meanwhile, SCL wear was discontinued for 1 month in a group of long-term wearers. Tear samples were then collected on days 1, 7, 14, 28, and 56 after treatment. Repeated-measures analysis of variance and Friedman’s test with post-hoc statistical analysis were used to evaluate biomolecular changes (HIF-1α concentration, LDH, and MDH activities) in both groups.</p> <p><strong>RESULTS </strong>A total of 14 subjects (28 eyes) were enrolled in each group. In new wearers, there was a significant decrease in MDH level (<em>p</em> = 0.010) and no effect on HIF-1α level. In long-term wearers, HIF-1α and LDH levels tended to decrease (<em>p</em> = 0.054). A significant decrease on MDH level was noted on days 7 (<em>p</em> = 0.003), 14 (<em>p</em> = 0.026), and 28 (<em>p</em>&lt;0.010). Long-term wearers had a higher LDH baseline level than new wearers (<em>p</em> = 0.04).</p> <p><strong>CONCLUSIONS</strong> Corneal hypoxia was not proven after 2 months of SCL wear using biomarkers. However, LDH and MDH activities in tears were found to be decline after SCL discontinuation.</p&gt;}, number={4}, journal={Medical Journal of Indonesia}, author={Rahayu, Tri and Gondhowiardjo, Tjahjono Darminto and Artini, Widya and Wanandi, Septelia Inawati}, year={2020}, month={Dec.}, pages={372-8} }