@article{Tarini_Wahid_Ibrahim_Yasmon_Djauzi_2010, title={Development of multiplex-PCR assay for rapid detection of Candida spp.}, volume={19}, url={http://mji.ui.ac.id/journal/index.php/mji/article/view/387}, DOI={10.13181/mji.v19i2.387}, abstractNote={<p><strong>Aim</strong>&nbsp;<em>Candida spp.</em> infection commonly occur in immunocompromised patients. Biochemical assay for identification of <em>Candida spp.</em> is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of <em>Candida spp.</em> had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify <em>Candida spp.</em></p> <p><strong>Methods&nbsp;</strong>Five Candida spp. isolates were cultured, identified with germ tube and API®20 C AUX (BioMerieux®SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay.</p> <p><strong>Results</strong> DNA detection limit by Multiplex-PCR assays for <em>C. albicans, C. tropicalis, C. parapsilosis, C. krusei</em> and <em>C. glabrata</em> were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.</p> <p><strong>Conclusion</strong> Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive andÂ&nbsp;specific assay for identification of <em>Candida spp.</em> <em><strong>(Med J Indones 2010; 19:83-7)</strong></em></p&gt;}, number={2}, journal={Medical Journal of Indonesia}, author={Tarini, Ni Made A. and Wahid, Mardiastuti H. and Ibrahim, Fera and Yasmon, Andi and Djauzi, Samsuridjal}, year={2010}, month={May}, pages={83-7} }