@article{Budianto_Firmansyah_Moenadjat_Jusuf_Soetikno_2021, title={<em>In vivo</em> model of Hirschsprung-associated enterocolitis using benzalkonium chloride}, volume={30}, url={http://mji.ui.ac.id/journal/index.php/mji/article/view/5339}, DOI={10.13181/mji.oa.215339}, abstractNote={<p><strong>BACKGROUND</strong> Hirschsprung-associated enterocolitis (HAEC) is a life-threatening complication of Hirschsprung’s disease. Studies using animal models on the pathogenesis of HAEC are limited. Thus, this study aimed to establish a rat model of HAEC using topical application of 0.1% benzalkonium chloride (BAC) in the sigmoid colon.</p> <p><strong>METHODS</strong> 55 male Sprague Dawley rats aged 10−12 weeks old were separated into 11 groups. The control group (n = 5) was euthanized on day-7, and the other 10 groups (n = 5 in each group) treated with 0.1% BAC in the sigmoid colon for 15 min to induce Hirschsprung’s disease were euthanized on day-7, -10, -12, -14, -17, -19, -21, -23, -25, and -28. The sigmoid colon was excised, fixed in formalin, and sectioned for histological examinations with hematoxylin and eosin staining. The degree of HAEC was compared within all groups.</p> <p><strong>RESULTS</strong> Rats that were sacrificed on day-7 to -12 showed the 1<sup>st</sup> degree or early HAEC, which was most likely caused by BAC application. The 2<sup>nd</sup> degree of HAEC occurred in rats that were sacrificed on day-14 that showed a macrophage infiltration in the sigmoid colon, thus fulfilled the initial criteria for HAEC (<em>p</em> = 0.0025 <em>versus</em> control). The degree of enterocolitis increased with time, and the highest degree was found in rats that were sacrificed on day-28 (<em>p</em>&lt;0.001 <em>versus</em> control).</p> <p><strong>CONCLUSIONS </strong>Topical application of 0.1% BAC for 15 min was successfully produced HAEC model in rats, which was occurred on day-14 after the application. This model provides a useful resource for further research on the pathogenesis of HAEC.</p&gt;}, number={4}, journal={Medical Journal of Indonesia}, author={Budianto, Iskandar Rahardjo and Firmansyah, Agus and Moenadjat, Yefta and Jusuf, Ahmad Aulia and Soetikno, Vivian}, year={2021}, month={Aug.}, pages={239-44} }