TY - JOUR AU - Reni Paramita AU - Mohamad Sadikin AU - Noorwati Sutandyo AU - Septelia I. Wanandi PY - 2014/10/15 Y2 - 2024/03/28 TI - Effect of hypoxia-inducible factor-1α induction by CoCl 2 on breast cancer cells survival: influence of cytochrome-c and survivin JF - Medical Journal of Indonesia JA - Med J Indones VL - 23 IS - 3 SE - Basic Medical Research DO - 10.13181/mji.v23i3.933 UR - http://mji.ui.ac.id/journal/index.php/mji/article/view/933 AB - Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.Methods: Breast cancer cell lines T47D were induced by CoCl 2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilitiesResults: HIF-1α induction by CoCl2 causes the increase of protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability.Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin. ER -