Bryophyllum pinnatum leaves ethanol extract inhibit maturation and promote apoptosis of systemic lupus erythematosus BALB/c mice B cells

  • Kusworini Handono Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang
  • Tri W.I. Dantara Biomedical Sciences Master Program, Faculty of Medicine, Universitas Brawijaya, Malang
  • Elvira S. Dewi Nursing Program, Faculty of Medicine, Universitas Brawijaya, Malang
  • Mirza Z. Pratama Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya, Malang
  • Nurdiana Nurdiana Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang
Keywords: B cells, Bryophyllum pinnatum, pristane, SLE
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Background: B cells play a key role in systemic lupus erythematosus (SLE). Targeting B cells as SLE therapy is a plausible approach. This study investigated the potential effects of Bryophyllum pinnatum leaves with ethanol extract in decreasing percentages of maturation, increasing percentages of apoptosis, and decreasing NF-κB p65 expressions of SLE BALB/c mice B cells.

Methods: Culturing B cells from pristane induced SLE BALB/c mice's spleen will resulted in this in vitro study. B cells were activated by BAFF, LPS, IL-4, and anti-CD40 yielding CD19+ >80%. B cells were cultured by adding those stimulants with and without B. pinnatum leaves (0, 0.02, 0.1, or 0.5 µg/ml) for 72 hours at 37°C. Flow cytometry was performed to determine The Percentages of maturation (CD19+CD38+) and apoptosis (Annexin V+PI+) of B cells. Further analysis to determine the expressions of transcription factor of maturation and apoptosis of B cells, NF-κB p65, were performed using immunocytochemistry. Data were analyzed using SPSS version 22.

Results: Flow cytometry assay showed significant decrease in percentages of maturation of B cells in all doses and significant increase in percentage of apoptosis of B cells in dose 0.5 µg/ml. Immunocytochemistry results showed significant decrease expressions of NF-κB p65 in all doses. Percentages of maturation, apoptosis, and expressions of NF-κB p65 of B cells were significantly correlated.

Conclusion: This in vitro study revealed that B. pinnatum leaves with ethanol extract decreased the percentages of maturation, increased the percentage of apoptosis, and decreased NF-κB p65 expressions of SLE BALB/c mice B cells significantly.


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How to Cite
Handono K, Dantara TW, Dewi ES, Pratama MZ, Nurdiana N. <em>Bryophyllum pinnatum</em&gt; leaves ethanol extract inhibit maturation and promote apoptosis of systemic lupus erythematosus BALB/c mice B cells. Med J Indones [Internet]. 2018Feb.14 [cited 2024Jul.20];26(4):253-60. Available from:
Basic Medical Research