Study of genetic diversity of Salmonella typhi using pulsed-field gel electrophoresis

Lucky H. Moehario; Retno K. Soemanto

doi:10.13181/mji.v10i3.25

Study of genetic diversity of Salmonella typhi using pulsed-field gel electrophoresis

Authors

Lucky H. Moehario
Retno K. Soemanto

DOI:

https://doi.org/10.13181/mji.v10i3.25

Keywords:

typhoid fever, sporadic cases, Xbal, cluster analysis

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Abstract

DNA genomes of Salmonella typhi (S. typhi), which were isolated from sporadic typhoid fever cases who were hospitalized in Persahabatan Hospital, Jakarta, during the 1^st semester of 1998, were examined for their genetic diversities. Pulsed-field gel electrophoresis (PFGE) of genomic DNA digested with Xbal was performed for 25 isolates. Electrophoresis patterns of most isolates varied and 18 PFGE types were identified. Cluster analysis showed that all isolates originated from two main groups; and at ≥84% level of similarity, 7 clusters were found. Thus, the results showed that genetic diversity of S. typhi was considerably high, and that S. typhi isolated from sporadic typhoid fever cases were derived from multiple clones. (Med J Indones 2001; 10: 158-63)

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Author Biographies

Lucky H. Moehario

Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia

Retno K. Soemanto

Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia

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Published

2001-08-01

How to Cite

Moehario LH, Soemanto RK. Study of genetic diversity of Salmonella typhi using pulsed-field gel electrophoresis. Med J Indones [Internet]. 2001Aug.1 [cited 2024Dec.22];10(3):158-63. Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/25

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Issue

Vol. 10 No. 3 (2001): July-September

Section

Basic Medical Research

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