Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

  • Seruni K.U. Freisleben
  • Jenny Hidayat
  • Hans-Joachim Freisleben
  • Soehardjo Poertadji
  • Budi Kurniawan
  • Na P. Bo
  • Sri Handayani
  • Pustika A. Wahidiyat
  • Rondang R. Soegianto
Keywords: electron paramagnetic resonance spectroscopy, erythrocyte membrane, lipoproteins, oxidative stress, thalassemia, plasma lipids
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Abstract

Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage.

Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10 controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status were measured.  Isolated  eryhtrocyte  membranes  were  investigated  with  electron  paramagnetic  resonance  (EPR)  spectroscopy  using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA.

Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. Vitamin  E,  group  C:  21.8  vs  T:  6.2  μmol/L)  and  reactive  thiols  (C:  144  vs.  T:  61  μmol/L)  were  considerably  lower  in  transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 μmol/L) and of tissue cell damage, i.e.,  high  transaminases  plasma  levels.  Non-transfused  thalassemia  patients  have  slight  signs  of  oxidative  stress,  but  no  signifi  cant  indication  of  cell  damage.  Erythrocyte  membrane  parameters  from  EPR  spectroscopy  differ  considerably  between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fl uidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized.

Conclusion: In  tranfusion-dependent  thalassemic  patients,  plasma  lipid  pattern  and  oxidative  stress  are  associated  with structural damage of isolated erythrocyte membranes as measured by EPR spectroscopy with lipid and protein-thiol spin labels. (Med J Indones 2011; 20:178-84)

Published
2011-08-01
How to Cite
1.
Freisleben SK, Hidayat J, Freisleben H-J, Poertadji S, Kurniawan B, Bo NP, Handayani S, Wahidiyat PA, Soegianto RR. Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta. Med J Indones [Internet]. 2011Aug.1 [cited 2021Aug.6];20(3):178-84. Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/448
Section
Basic Medical Research

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