Correlation of zinc plasma and IgM anti-PGL-1 levels among close contact of leprosy

Mohammad Z. Rahfiludin; Praba Ginandjar; Dina R. Pangestuti

doi:10.13181/mji.v21i3.500

Authors

Mohammad Z. Rahfiludin
Praba Ginandjar
Dina R. Pangestuti

DOI:

https://doi.org/10.13181/mji.v21i3.500

Keywords:

IgM anti PGL-1, close contact of leprosy, zinc plasma

Abstract

Background: Previous study showed leprosy patients have low zinc status. Yet the status of zinc in close-contact, which indicated by IgM anti-PGL1 level, have not determined. The aim of the study was to determine the association of zinc plasma and IgM anti-PGL-1 levels among close contact of leprosy patients in Indonesia.

Methods: This was an observational study. Subjects were 186 close-contact leprosy patients. Measurement of zinc plasma status used atomic absorption spectrophotometer while examination of IgM anti-PGL1 level used ELISA (Polyclonal rabbit anti human IgM/HRP/(Dako®).

Results: The average level of IgM anti-PGL1 and zinc plasma were 804 unit/mL (± 439.4) and 16.6 μmol/L (± 3.5) consecutively. There was significant correlation between zinc plasma and IgM anti-PGL1 (r = - 0.230; p = 0.002).

Conclusion: There is a significant correlation between zinc plasma and IgM anti-PGL1 in close contact of leprosy (Med J Indones. 2012;21:166-9)

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References

Kerr-Pontes LR, Montenegro AC, Barreto ML, Werneck GL, Feldmeier H. Inequality and leprosy in Northeast Brazil: an ecological study. Int J Epidemiol. 2004;33:262-9.

Ponnighaus JM, Fine PE, Sterne JA, Malema SS, Bliss L, Wilson RJ. Extended schooling and good housing conditions are associated with reduced risk of leprosy in rural Malawi. Int J Lepr Other Mycobact Dis.1994;62:345-52.

Rahfiludin MZ, Nugraheni SA, Ametati H, Prihatin A, Purwaningsih E. The difference of anti phenolic glycolipid-1 (PGL-1) immunoglobulin-M (IgM) level and nutritional intake in subclinical leprosy patients who reside at home and in the orphanage. Med J Indones. 2007;16(4):233-6.

Hotz C, Brown KH, editors. International Zinc Nutrition Consultative Group (IZiNCG) technical document #1: Tabel 2.Assosiation between zinc status and serological status in close contact of leprosyZinc StatusDeficientNormalTotalSero-positive32 (24.6 %)98 (75.4 %)130 (100.0 %)Sero-negative0 (0 %)56 (100.0 %)56 (100.0 %)assessment of the risk of zinc deficiency in populations and option for its control. Food Nutr Bull. 2004;25:S94-204.

Prasad AS. Zinc: mechanisms of host defense. J Nutr. 2007;137(5):1345-9.

Mathur NK, Sharma M, Mangal HN, Rai SM. Serum zinc level in subtypes of leprosy. Int J Lepr Other Mycobact Dis. 1984;52:327-30.

George J, Bathia VN, Balakrishnan S, Ramu G. Serum zinc/copper ratio in subtypes of leprosy and effect of oral zinc therapy on reactional states. Int J Lepr Other Mycobact Dis. 1991;59:20-4.

Saxena N, Sharma RP, Singh VS. Study of serum zinc level in leprosy. Indian J Lepr. 1988;60:556-61.

Izumi S. Subclinical infection by Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 1999;67(4 Suppl):S67-71.

Barreto JG, Guimaraes LD, Leao MR, Ferreira DV, Lima RA, Salgado CG. Anti-PGL-1 seroepidemiology in leprosy cases: household contacts and school children from a hyperendemic municipality of the Brazilian Amazon. Lepr Rev. 2011;82:358-70.

Sandström B. Diagnosis of zinc deficiency and excess in individuals and populations. Food Nutr Bull. 2001;22:133-7.

Cuevas LE, Koyanagi A. Zinc and infection: a review. Ann Trop Paediatr. 2005;25:149-60.13. Gibson RS. Principles of nutritional assessment. 2nd ed. Oxford: Oxford University Press; 2005.

Harboe M. Overview of host – parasite relation. In : Hastings RC. Leprosy. Churchil Livingstone. Edinburg; 1994.

Scollard DM, Adams LB, Gillis TP, Krahenbuhl JP, Truman RW, Williams DL. The continuing challenges of leprosy. Clin Microbiol Rev. 2006;19(2):338-81.

Maeda SM, Rotta O, Michalany NS, Camargo ZP, Sunderkötter C, Yamashita JT. Comparison between anti PGL-1 serology and Mitsuda reaction: clinical reading, microscopic findings and immunohistochemical analysis. Lepr Rev. 2003;74:263-74.

Oskam L, Slim E, Bührer-Sékula S. Serology: recent developments, strengths, limitations and prospects: a state of the art overview. Lepr Rev. 2003;74:196-205.

Solomons NW. Mild human zinc deficiency produces an imbalance between cell-mediated and humoral immunity. Nutr Rev. 1998;56(1): 27-8.

Prasad AS. Effects of zinc deficiency on TH1 and TH2 cytokine shifts. J Infect Dis. 2000;182;S62-8.

Rahfiludin MZ, Wirjatmadi B, Agusni I, Dahlan YP. Zinc supplementation could modulate T cell to maintain interleukin-2 level in seropositive contact of leprosy patients. Med J Indones. 2011;20:201-4.

Haase H,Rink L. Functional significance of zinc – related signaling pathways in immune cells. Annu Rev Nutr. 2009;29:133-52.r = -0.230, Px2 = 0.002

Chattree V, Khanna N, Rao DN. Alterations in T cell signal transduction by M. leprae antigens is associated with downregulation of second messengers PKC, calcium, calcineurin, MAPK and various transcription factors in leprosy patients. Mol Immunol. 2007;44(8):2066-77.

Prasad AS. Zinc: role in immunity, oxidative stress and chronic inflammation. Curr Opin Clin Nutr Metab Care. 2009;12:646-52.

Russell DG, Mwandumba HC, Rhoades EE. Mycobacterium and the coat of many lipids. J Cell Biol. 2002;158(3):421-6.