RAGE and HMGB1 expressions in fetal membranes of premature rupture of membranes patients

  • Ketut Edy Sudiarta Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Hang Tuah, Dr. Ramelan Central Naval Hospital, Surabaya, Indonesia
  • Cindy Jennilyn Candra Faculty of Medicine, Universitas Hang Tuah, Surabaya, Indonesia
  • Joan Khan Faculty of Medicine, Universitas Hang Tuah, Surabaya, Indonesia https://orcid.org/0000-0003-4249-9391
  • Rahadianto Department of Clinical Pathology, Faculty of Medicine, Universitas Hang Tuah, Dr. Ramelan Central Naval Hospital, Surabaya, Indonesia
  • Fitri Handajani Department of Biochemistry, Faculty of Medicine, Universitas Hang Tuah, Dr. Ramelan Central Naval Hospital, Surabaya, Indonesia https://orcid.org/0000-0001-8302-0151
Keywords: fetal membranes, high mobility group box protein 1, immunohistochemistry, premature rupture of membranes, receptor for advanced glycation end products
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Abstract

BACKGROUND Premature rupture of membranes (PROM) often occurs in pregnancy. The fetal membrane weakening is caused by inflammation involving receptor activation for advanced glycation end-products (RAGE) and high mobility group box protein 1 (HMGB1). The associations between RAGE and HMGB1 with PROM are rarely studied. Hence, this study aimed to determine those associations in fetal membranes with PROM occurrence.

METHODS This case-control study was conducted at Dr. Ramelan Central Naval Hospital, Surabaya, Indonesia, from August to November 2019. The subjects, determined using a non-probability sampling method (a saturated sample), were divided into PROM and normal pregnancy with intact fetal membranes (control) groups. Fetal membrane specimens were collected during vaginal and cesarean section deliveries. The expressions of RAGE and HMGB1 were determined using the immunohistochemical method and further analyzed using the Mann–Whitney U test.

RESULTS The expression of RAGE in fetal membranes with PROM was significantly higher than the control (52.74% versus 14.9% expression/mm2, p<0.001), as well as the expression of HMGB1 (45.9% versus 8.5% expression/mm2, p<0.001).

CONCLUSIONS The higher expressions of RAGE and HMGB1 in fetal membranes were associated with PROM.

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Published
2022-09-22
How to Cite
1.
Sudiarta KE, Candra CJ, Khan J, Rahadianto, Handajani F. RAGE and HMGB1 expressions in fetal membranes of premature rupture of membranes patients. Med J Indones [Internet]. 2022Sep.22 [cited 2022Oct.3];1(1). Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/6099
Section
Clinical Research