Bone growth evaluation in collagen-hydroxyapatite implant locations using digital radiography: an animal model
DOI:
https://doi.org/10.13181/mji.oa.237051Keywords:
bone growth, bone implant, collagen, digital radiography, hydroxyapatiteAbstract
BACKGROUND Digital radiography has been used to evaluate the progress of bone growth with a collagen-hydroxyapatite implant in rabbit tibias. This study aimed to introduce digital radiography methods that provide comprehensive data availability for continuous information retrieval from the implant preparation to the cultivation period.
METHODS 38 digital radiographs were divided into 3 treatment groups, namely a single defect without implant (control), single-implant, and three-implant. Radiographic acquisitions were performed at preparation time and post-implantation from 0 to 56 days. Observations were concentrated on the implantation site, followed by creating a lateral profile. The prediction of implantation growth was determined using relative bone density (RBD) percentage.
RESULTS Based on the profile, the recovery process consisted of implant absorption and new bone tissue deposition. The absorption process was highly influenced by the defect size. In the control and single-implant groups, regardless of the different recovery processes, similar recovery results were observed 56 days post-implantation, with an RBD value of approximately 90%. Meanwhile, the three-implant group only had an RBD value of 62%.
CONCLUSIONS Radiography can evaluate absorption and new bone growth during implantation in New Zealand white rabbits. Radiographs, which can be obtained at any time during cultivation, offered more information on the recovery implantation process than the other method that relies on data obtained after sacrificing the animals.
Downloads
References
Wong JY, Bronzino JD, Peterson DR, editors. Biomaterials: principles and practices. 2nd ed. Boca Raton: CRC Press; 2013. https://doi.org/10.1201/b13687
Knop C, Sitte I, Canto F, Reinhold M, Blauth M. Successful posterior interlaminar fusion at the thoracic spine by sole use of beta-tricalcium phosphate. Arch Orthop Trauma Surg. 2006;126(3):204-10. https://doi.org/10.1007/s00402-006-0107-8
Mahyudin F. [Bone graft and bone replacement materials: characteristics and clinical application strategies]. Utomo DN, editor. Surabaya: Airlangga University Press; 2018. Indonesian.
Hartono SA. [Intradermal Irritation test and radiography density of HA:Ce-Zn bone graft in femoral bone and muscle of Sprague Dawley rats] [thesis]. Bogor: IPB Unversity; 2021. Indonesian.
Rémi E, Khelil N, Di Centa I, Roques C, Ba M, Medjahed-Hamidi F, et al. Pericardial processing: challenges, outcomes and future prospects, biomaterials science and engineering. Oxford: INTECH Open Access Publisher; 2011. p. 437-56. In: Pignatello R, editors. Chapter 22, Biomaterials science and engineering. https://doi.org/10.5772/24949
Bohner M, Galea L, Doebelin N. Calcium phosphate bone graft substitutes: failures and hopes. J Eur Ceram Soc. 2012;32(11):2663-71. https://doi.org/10.1016/j.jeurceramsoc.2012.02.028
Moore WR, Graves SE, Bain GI. Synthetic bone graft substitutes. ANZ J Surg. 2001;71(6):354-61. https://doi.org/10.1046/j.1440-1622.2001.02128.x
Arifin A. [Development of hydroxyapatite/titanium compiste as implants using metal injection molding (MIM) technology]. Palembang: Unsri Press; 2017. p. 1-82. Indonesian.
Azami M, Tavakol S, Samadikuchaksaraei A, Hashjin MS, Baheiraei N, Kamali M, et al. A Porous hydroxyapatite/gelatin nanocomposite scaffold for bone tissue repair: in vitro and in vivo evaluation. J Biomater Sci Polym Ed. 2012;23(18):2353-68. https://doi.org/10.1163/156856211X617713
Liu C. Collagen-hydroxyapatite composite scaffolds for tissue engineering hydroxyapatite (Hap) for biomedical applications. Elsevier Ltd.; 2015. p. 211-34. https://doi.org/10.1016/B978-1-78242-033-0.00010-9
Yang X, Li Y, Huang Q, Yang J, Shen B, Pei F. Evaluation of a biodegradable graft substitute in rabbit bone defect model. Indian J Orthop. 2012;46(3):266-73. https://doi.org/10.4103/0019-5413.96371
Purwanti S. [Radiography evaluation of hydroxyapatite-chitosan (HA-C) and hydroxyapatite-tricalcium phosphate (HA-TCP) bone implant in sheep as animal model for human] [thesis]. Bogor: IPB University; 2010. Indonesian.
Geiger M, Blem G, Ludwig A. Evaluation of ImageJ for relative bone density measurement and clinical application. J Oral Health Craniofac Sci. 2016;1:012-21. https://doi.org/10.29328/journal.johcs.1001002
Lee SW, Hahn BD, Kang TY, Lee MJ, Choi JY, Kim MK, et al. Hydroxyapatite and collagen combination-coated dental implants display better bone formation in the peri-implant area than the same combination plus bone morphogenetic protein-2-coated implants, hydroxyapatite only coated implants, and uncoated implants. J Oral Maxillofac Surg. 2014;72(1):53-60. https://doi.org/10.1016/j.joms.2013.08.031
Hoshi M, Taira M, Sawada T, Hachinohe Y, Hatakeyama W, Takafuji K, et al. Preparation of collagen/hydroxyapatite composites using the alternate immersion method and evaluation of the cranial bone-forming capability of composites complexed with acidic gelatin and b-FGF. Materials (Basel). 2022;15(24):8802. https://doi.org/10.3390/ma15248802
Minardi S, Taraballi F, Cabrera FJ, Van Eps J, Wang X, Gazze SA, et al. Biomimetic hydroxyapatite/collagen composite drives bone niche recapitulation in a rabbit orthotopic model. Mater Today Bio. 2019;2:100005. https://doi.org/10.1016/j.mtbio.2019.100005
Windolf M, Varjas V, Gehweiler D, Schwyn R, Arens D, Constant C, et al. Continuous implant load monitoring to assess bone healing status-evidence from animal testing. Medicina (Kaunas). 2022;58(7):858. https://doi.org/10.3390/medicina58070858
Published
How to Cite
Issue
Section
License
Authors who publish with Medical Journal of Indonesia agree to the following terms:
- Authors retain copyright and grant Medical Journal of Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial License that allows others to remix, adapt, build upon the work non-commercially with an acknowledgment of the work’s authorship and initial publication in Medical Journal of Indonesia.
- Authors are permitted to copy and redistribute the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Medical Journal of Indonesia.
