Comparison of stingless bee honey and silver sulfadiazine on diabetic wound healing in rat models

Nanang Miftah Fajari; Agung Pranoto; David Sontani Perdanakusuma; Muhammad Darwin Prenggono; Mohammad Rudiansyah; Hendra Wana Nur'amin; Yulia Syarifa; Nuvita Hasrianti; Imelda Nita Saputri; Muhammad Irawan Afrianto; Annisa Halida Husna

doi:10.13181/mji.oa.257974

Authors

Nanang Miftah Fajari Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia
Agung Pranoto Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Surabaya, Indonesia
David Sontani Perdanakusuma Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Surabaya, Indonesia
Muhammad Darwin Prenggono Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia
Mohammad Rudiansyah Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia
Hendra Wana Nur'amin Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia; Department of Pharmacology, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Banjarmasin, Indonesia
Yulia Syarifa Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia
Nuvita Hasrianti Internal Medicine Residency Program, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia
Imelda Nita Saputri Internal Medicine Residency Program, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia
Muhammad Irawan Afrianto Internal Medicine Residency Program, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia
Annisa Halida Husna Internal Medicine Residency Program, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Ulin Hospital, Banjarmasin, Indonesia

DOI:

https://doi.org/10.13181/mji.oa.257974

Keywords:

diabetic foot, honey, silver sulfadiazine, wound healing

Abstract

BACKGROUND Diabetic foot ulcers (DFUs) are a major health concern in Indonesia. Adjuvant therapies may improve healing by avoiding secondary infections, promoting angiogenesis, and supporting oxygen circulation. This aimed to evaluate the effect of stingless bee honey (SBH) from Heterotrigona itama on diabetic wound size in rats (Rattus norvegicus), compared to silver sulfadiazine (SSD).

METHODS An experimental study was conducted on 13 diabetic wounds in streptozotocin-induced diabetic rats treated with three types of therapies: SSD (n = 5), pure SBH (n = 5), and SBH with 20% water content (n = 3). The study initially involved 21 rats, but eight died during the diabetes modeling and wound observation phases, presumably due to hyperglycemia. Baseline characteristics did not differ significantly across the groups.

RESULTS SBH with 20% water content and pure SBH reduced wound size by 95.1% and 92.1%, outperforming SSD (77.4%), with all therapies showing statistically significant improvement (p<0.05). However, the differences between groups were not statistically significant (p = 0.162).

CONCLUSIONS Topically applied SBH is a potential natural therapeutic agent for diabetic wounds, in addition to standard treatment such as SSD.

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