Effect of hypoxia-inducible factor-1α induction by CoCl 2  on  breast cancer cells survival: influence of cytochrome-c and  survivin

Reni Paramita; Mohamad Sadikin; Noorwati Sutandyo; Septelia I. Wanandi

doi:10.13181/mji.v23i3.933

Authors

Reni Paramita Doctoral student in Biomedical Study, Faculty of Medicine, Universitas Indonesia, Jakarta
Mohamad Sadikin Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta
Noorwati Sutandyo Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta
Septelia I. Wanandi Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta

DOI:

https://doi.org/10.13181/mji.v23i3.933

Keywords:

apoptosis, cell viability, cytochrome-c, hypoxia-inducible factor-1α, survivin

Abstract

Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.

Methods: Breast cancer cell lines T47D were induced by CoCl 2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities

Results: HIF-1α induction by CoCl₂ causes the increase of protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability.

Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.

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