Basal follicle stimulating hormone and leptin on the day of hCG administration predict successful fertilization in <em>in vitro</em> fertilization

Authors

  • Andon Hestiantoro Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
  • Budi Wiweko Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
  • Robert H. Purwaka Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta

DOI:

https://doi.org/10.13181/mji.v25i1.1215

Keywords:

basal FSH, IVF, leptin serum, oocyte ratio

Abstract

Background: Successful pregnancy in in vitro fertilization (IVF) program depends on multiple factors. This study aimed to determine whether age, body mass index (BMI), basal follicle stimulating hormone (FSH), estradiol, and leptin on the day of trigger ovulation with human chorionic gonadotropin (hCG) might be used as predictor for successful oocyte fertilization in in vitro fertilization (IVF) program.

Methods: This is a cross sectional study conducted in Yasmin Fertility Clinic, Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Forty participating patients underwent IVF program, excluding smokers, patients with diabetic, morbid obesity, and severe oligospermia or azoospermia. Age, BMI, basal FSH, estradiol, leptin on the day of hCG administration, oocyte count on oocyte retrieval, the number of mature oocyte, and fertility rate were analyzed using bivariate and multivariate analysis to determine which eligible factors play role in predicting the successful of fertilization.

Results: Significant correlation was found between basal FSH level and serum leptin/oocyte ratio on the day of hCG administration with successful fertilization. We found probability formula as follows: 1/(1+exp –(6.2 - 0.4(leptin serum/oocyte ratio) - 0.8(basal FSH)), with 77.8% sensitivity, 77.8% specificity, and AUC levels of 85.6% indicating strong predictability. Probability of successful fertilization related to basal FSH level of 5.90 mIU/mL and leptin serum/oocyte ratio of 3.98.

Conclusion: The formula consisting of basal FSH and leptin serum/oocyte ratio on the day of trigger ovulation was capable in predicting the probability of successful fertilization in IVF procedure.

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References

Toma´´s C, Tikkinen K, Tuomivaara L, S.Tapanainen J, Martikainen H. The degree of difficulty of embryo transfer is an independent factor for predicting pregnancy. Hum Reprod. 2002;17(10):2632–5. http://dx.doi.org/10.1093/humrep/17.10.2632

Practice Committee of Society for Assisted Reproductive T, Practice Committee of American Society for Reproductive Medicine. Guidelines on number of embryos transferred. Fertil Steril. 2008;90(5 Suppl):S163–4. http://dx.doi.org/10.1016/j.fertnstert.2008.08.053

Marteil G, Richard-Parpaillon L, Kubiak JZ. Role of oocyte quality in meiotic maturation and embryonic development. Reprod Biol. 2009;9(3):203–24. http://dx.doi.org/10.1016/S1642-431X(12)60027-8

Craig J, Zhu H, Dyce PW, Petrik J, Li J. Leptin enhances oocyte nuclear and cytoplasmic maturation via the mitogen-activated protein kinase pathway. Endocrinology. 2004;145(11):5355–63. http://dx.doi.org/10.1210/en.2004-0783

Matorras R, Meabe A, Mendoza R, Prieto B, Ramon O, Mugica J, et al. Human chorionic gonadotropin (hCG) plasma levels at oocyte retrieval and IVF outcomes. Journal of assisted reproduction and genetics. 2012;29(10):1067–71. http://dx.doi.org/10.1007/s10815-012-9826-7

Beretsos P, Partsinevelos GA, Arabatzi E, Drakakis P, Mavrogianni D, Anagnostou E, et al. "hCG priming" effect in controlled ovarian stimulation through a long protocol. Reprod Biol Endocrinol. 2009;7:91. http://dx.doi.org/10.1186/1477-7827-7-91

Andersen CY, Ezcurra D. Human steroidogenesis: implications for controlled ovarian stimulation with exogenous gonadotropins. Reprod Biol Endocrinol. 2014;12:128. http://dx.doi.org/10.1186/1477-7827-12-128

Excellence NIfC. Fertility: assessment and treatment for people with fertility problems. London: RCOG Press; 2004.

Patrizio P, Vaiarelli A, Levi Setti PE, Tobler KJ, Shoham G, Leong M, et al. How to define, diagnose and treat poor responders? Responses from a worldwide survey of IVF clinics. Reprod Biomed Online. 2015;30(6):581–92. http://dx.doi.org/10.1016/j.rbmo.2015.03.002

Bancsi LF, Huijs AM, den Ouden CT, Broekmans FJ, Looman CW, Blankenstein MA, et al. Basal follicle-stimulating hormone levels are of limited value in predicting ongoing pregnancy rates after in vitro fertilization. Fertil Steril. 2000;73(3):552–7. http://dx.doi.org/10.1016/S0015-0282(99)00552-X

Anifandis G, Koutselini E, Stefanidis I, Liakopoulos V, Leivaditis C, Mantzavinos T, et al. Serum and follicular fluid leptin levels are correlated with human embryo quality. Reproduction. 2005;130(6):917–21. http://dx.doi.org/10.1530/rep.1.00705

Mantzoros CS, Cramer DW, Liberman RF, Barbieri RL. Predictive value of serum and follicular fluid leptin concentratrions during assisted reproductive cycles in normal women and in women with the polycyistics ovarian syndrome. Hum Reprod. 2000;15(3):539–44. http://dx.doi.org/10.1093/humrep/15.3.539

Published

2016-04-15

How to Cite

1.
Hestiantoro A, Wiweko B, Purwaka RH. Basal follicle stimulating hormone and leptin on the day of hCG administration predict successful fertilization in &lt;em&gt;in vitro&lt;/em&gt; fertilization. Med J Indones [Internet]. 2016Apr.15 [cited 2024Nov.6];25(1):39-43. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1215

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Section

Clinical Research
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