Effect of zinc supplementation on triglyceride and malondialdehyde levels: study on diabetic Wistar rats induced with streptozotocin

Resty Ryadinency, Suharyo Hadisaputro, Banundari Rachmawati

DOI: https://doi.org/10.13181/mji.v27i2.1417


Background: Diabetes mellitus is associated with increased blood glucose and triglyceride levels, which can lead to an oxidative stress. Zinc (Zn) is a micronutrient that has antioxidant properties and involved in lipid and glucose metabolism. This study aimed to evaluate the effect of zinc on the levels of fasting blood glucose (FBG), triglycerides (TG), and malondialdehyde (MDA) in male diabetic Rattus norvegicus Wistar rats.

Methods: Diabetes was induced by intraperitoneal (i.p.) injection of 40 mg/kg BW streptozotocin (STZ) and confirmed by FBG level higher than 200 mg/dL after 2 weeks. The rats were randomly divided into three groups: control group (STZ), treatment I (STZ + zinc 5 mg/kg BW), and treatment II (STZ + zinc 10 mg/kg BW). Zinc was administered by oral gavage for 30 days. At the end of the experiment, levels of FBG, TG, and MDA were measured. Data were analyzed using paired t-test or Wilcoxon test as appropriate.

Results: Supplementation of 5 mg/kg zinc significantly decreased the levels of FBG (pre-intervention: 328.95±70.90 mg/dL, post-intervention: 144.35±34.27 mg/dL, p<0.05), TG (pre-intervention: 252.48±26.30 mg/dL, post-intervention: 147.90±12.18 mg/dL, p<0.05), and MDA (pre-intervention: 12.11±6.46 nm/mL, post-intervention: 4.75±2.34 nm/mL, p<0.05). Moreover, supplementation of 10 mg/kg zinc decreased the levels of TG (pre-intervention: 275.62±56.25nm/mL, post-intervention: 165.58±22.63 nm/mL, p<0.05) and MDA (pre-intervention: 13.08±6.60 nm/mL, post-intervention: 5.08±2.40 nm/mL, p<0.05).

Conclusion: Supplementation of zinc significantly reduced the levels of FBG, TG, and MDA in diabetic rats.


fasting blood glucose, diabetes mellitus, MDA, triglycerides, zinc

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  1. Arthur CG, John EH. Text book of medical physiology. 11th ed. USA: Elsevier Saunder. 2006. p. 974.
  2. WHO Media center. Diabetes fact. World Health Organization. 2013. p. 312.
  3. Moussa SA. Oxidative stress in diabetes mellitus. Rom J Biophys. 2008;18(5):225–36.
  4. Ramakrishna V, Jailkhani R. Evaluation of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagn Pathol. 2007;2:22. https://dx.doi.org/10.1186/1746-1596-2-22.
  5. Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol. 2004;14(2):231–55. https://doi.org/10.1038/sj.bjp.0705776
  6. Andallu B, Vinay Kumar AV, Varadacharyulu N Ch. Lipid abnormalities in streptozotocin-diabetes: amelioration by Morus indica L. cv Suguna leaves. Int J Diabetes Dev Ctries. 2009;29(3):123–8. https://dx.doi.org/10.4103/0973-3930.54289.
  7. Shams ME, Al-Gayyar MM, Barakat EA. Type 2 diabetes mellitus-induced hyperglycemia in patients with NAFLD and normal LFTs: relationship to lipid profile, oxidative stress and pro-inflammatory cytokines. Sci Pharm. 2011;79(3):623–34. http://dx.doi.org/10.3797/scipharm.1104-21
  8. Manohar SM, Vaikasuvu SR, Deepthi K, Sachan A, Narasimha SR. An association of hyperglycemia with plasma malondialdehyde and atherogenic lipid risk factor in newly diagnosed type 2 diabetic patients. J Res Med Sci. 2013;18(2):89–93.
  9. Sheikhpour R, Jalali B, Yaghmaei P, Afkhami-Ardekani M, Rashidi M. Comparison of two supplementary zinc doses on lipid peroxidation in diabetic patients. Iranian J Diabetes Obes. 2010;2(2):17–22.
  10. Bicer M, Akil M, Sivrikaya A, Kara E, Baltaci AK, Mogulkoc R. Effects of zinc supplementation on the distribution of various elements in the serum of diabetic rats subjected to an acute swimming exercise. J Physiol Biochem. 2011;67(4):511–7. https://dx.doi.org/10.1007/s13105-011-0096-0
  11. Vardatsikos G, Pandey NR, Srivastava AK. Insulino- mimetic and anti-diabetic effects of zinc. J Inorg Biochem. 2013;120:8–17. https://doi.org/10.1016/j.jinorgbio.2012.11.006
  12. Ranasinghe P, Wathurapatha WS, Ishara MH, Jayawardana R, Galappatthy P, Katulanda P, et al. Effect of zinc supplementation on serum lipids: a systematic review and meta-analysis. Nutr Metab (Lond). 2015;12:26. https://doi.org/10.1186/s12986-015-0023-4
  13. Kechrid Z, Derai EH, Layachi N. The beneficial effect of vitamin E supplementation on zinc status, carbohydrate metabolism, transaminases and alkaline phosphatase activities in alloxan diabetic rats fed on zinc deficiency diet. Intl J Diabetes Metab. 2007;15:46–50.
  14. de Carvalho EN, Ferreira LM, de Carvalho NA, Abla LE, Liebano RE. Viability of a random pattern dorsal skin flap, in diabetic rats. Acta Cir Bras. 2005;20(3):225–8. http://dx.doi.org/10.1590/S0102-86502005000300006
  15. Zhu K, Nie S, Li C, Huang J, Hu X, Li W, et al. Antidiabetic and pancreas-protective effects of zinc threoninate chelate in diabetic rats may be associated with its antioxidant stress ability. Biol Trace Elem Res. 2013;153(1–3):291–8. https://dx.doi.org/10.1007/s12011-013-9675-y.
  16. Lin CC, Huang HH, Hu CW, Chen BH, Cheng IW, Chao YY, et al. Trace elements, oxidative stress and glycemic control in young people with type 1 diabetes mellitus. J Trace Elem Med Biol. 2014;28(1):18–22. https://doi.org/10.1016/j.jtemb.2013.11.001
  17. Capdor J, Foster M, Petocz P, Samman S. Zinc and glycemic control: a meta-analysis of randomised placebo controlled supplementation trials in humans. J Trace Elem Med Biol. 2013;27(2):137–42. https://doi.org/10.1016/j.jtemb.2012.08.001
  18. Sheetz MJ, King GL. Molecular understanding of hypergycemia’s adverse effects for diabetic complications. JAMA. 2002;288(20);2579–88. https://dx.doi.org/10.1001/jama.288.20.2579.
  19. Kathleen MB, Peter AM. Metabolisme asigliserol and sfingolipid. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Biokimia harper. 27th ed. Jakarta: EGC. 2009. p. 217.
  20. Umrani RD, Paknikar KM. Zinc oxide nanoparticles show antidiabetic activity in streptozotocin-induced type 1 and 2 diabetic rats. Nanomedicine (Lond). 2012;9(1):89–104. https://dx.doi.org/10.2217/nnm.12.205
  21. Ghayour-Mobarhan M, Taylor A, New SA, Lamb DJ, Ferns GA. Determinants of serum copper, zinc and selenium in healthy subjects. Ann Clin Biochem. 2005;42(Pt 5):364–75. https://dx.doi.org/10.1258/0004563054889990
  22. Asri-Rezaei S, Tamaddonfard E, Ghasemsoltani-Momtaz B, Erfanparast A, Gholamalipour S. Effects of crocin and zinc chloride on blood levels of zinc and metabolic and oxidative parameters in streptozotocin-induced diabetic rats. Avicenna J Phytomed. 2014;5(5):403–12.
  23. Aly HF, Mantawy MM. Comparative effects of zinc, selenium and vitamin E or their combination on carbohydrate metabolizing enzymes and oxidative stress in streptozotocin induced-diabetic rats. Eur Rev Med Pharmacol Sci. 2012;16(1):66–78.
  24. DiSilvestro RA. Zinc in relation to diabetes and oxidative disease. J Nutr. 2000;130(5):1509S–11S. https://doi.org/10.1093/jn/130.5.1509S

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