Soybean extract increases telomerase reverse transcriptase protein expression in pancreatic β-cells of diabetes mellitus-induced rats

  • Muhammad Samsul Mustofa Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Biology, Faculty of Medicine, Universitas Yarsi, Jakarta, Indonesia https://orcid.org/0000-0002-8043-3345
  • Franciscus Dhyanagiri Suyatna Department of Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Mohamad Sadikin Department of Biochemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Dwi Ari Pujianto Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Aan Royhan Department of Histology, Faculty of Medicine, Universitas Yarsi, Jakarta, Indonesia
  • Kenconoviati Suwardji Department of Histology, Faculty of Medicine, Universitas Yarsi, Jakarta, Indonesia
DOI: https://doi.org/10.13181/mji.v28i3.1732
Keywords: diabetes mellitus, rats, soybeans, telomerase reverse transcriptase
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Abstract

BACKGROUND A number of studies on the benefits of soybean (Glycine max (L.) Merr) in the treatment of diabetes mellitus (DM) have already been conducted; however, the effects of soybean extracts on telomerase reverse transcriptase (TERT) expression in improving telomerase activity in pancreatic cells is currently unknown. This study was aimed to evaluate the effects of soybean extracts on TERT protein expression in the pancreatic β-cells of rats with DM. 

METHODS Sixty male Sprague-Dawley rats were randomly divided into six groups: (1) negative control (N); (2) DM rats induced by alloxan (DMA); (3) DM rats with glibenclamide (DMG; positive control); (4) DM rats with 1 mg/kgBW/day soybean extract (DM1E); (5) DM rats with 5 mg/kgBW/day soybean extract (DM5E); (6) DM rats with 25 mg/kgBW/day soybean extract (DM25E). The treatments were carried out over 28 days. The measured variables included fasting blood glucose (FBG) level, TERT protein expression, and the number of pancreatic β-cells. 

RESULTS All parameters were measured against the diabetes control group. The FBG levels in rats DM1E, DM5E, and DM25E were significantly reduced on the 28th day (p < 0.05). TERT protein expression and the number of pancreatic β-cells (DM25E) also showed significant improvements compared to DM rats (p < 0.05). 

CONCLUSIONS Soybean extracts can increase TERT protein expression in pancreatic β-cells in diabetes-induced rats. 

References

  1. Kaneto H, Katakami N, Matsuhisa M, Matsuoka TA. Role of reactive oxygen species in the progression of type 2 diabetes and atherosclerosis. Mediators Inflamm. 2010;2010:453892. https://doi.org/10.1155/2010/453892

  2. Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: a review. Int J Pharm Appl. 2010;1(1):38-45.

  3. Chaiyasut C, Kumar T, Tipduangta P, Rungseevijitprapa W. Isoflavone content and antioxidant activity of Thai fermented soybean and its capsule formulation. Afr J Biotechnol. 2010;9(26):4120-6.

  4. Zhang Z, Liew CW, Handy DE, Zhang Y, Leopold JA, Hu J, et al. High glucose inhibits glucose-6-phosphate dehydrogenase, leading to increased oxidative stress and β-cell apoptosis. FASEB J. 2010;24(5):1497-505. https://doi.org/10.1096/fj.09-136572

  5. Salpea KD, Talmud PJ, Cooper JA, Maubaret CG, Stephens JW, Abelak K, et al. Association of telomere length with type 2 diabetes, oxidative stress and UCP2 gene variation. Atherosclerosis. 2010;209(1):42-50. https://doi.org/10.1016/j.atherosclerosis.2009.09.070

  6. Kuhlow D, Florian S, von Figura G, Weimer S, Schulz N, Petzke KJ, et al. Telomerase deficiency impairs glucose metabolism and insulin secretion. Aging. 2010;2(10):650-8. https://doi.org/10.18632/aging.100200

  7. Zhu H, Belcher M, van der Harst P. Healthy aging and disease: role for telomere biology? Clin Sci (Lond). 2011;120(10):427-40. https://doi.org/10.1042/CS20100385

  8. Lipińska N, Rubiś B. Telomerase as a target in diagnosis and treatment of cancer in postmenopausal women. Przeglad Menopauzalny. 2013;17(6):478-83. https://doi.org/10.5114/pm.2013.39812

  9. Haendeler J, Hoffmann J, Diehl JF, Vasa M, Spyridopoulos I, Zeiher AM, et al. Antioxidants inhibit nuclear export of telomerase reverse transcriptase and delay replicative senescence of endothelial cells. Circ Res. 2004;94(6):768-75. https://doi.org/10.1161/01.RES.0000121104.05977.F3

  10. Valsecchi AL, Franchi S, Panerai AE, Rossi R, Sacerdote P, Colleoni M. The soy isoflavone genistein reverses oxidative and inflammatory state, neuropathic pain, neurotrophic and vasculature deficits in diabetes mouse model. Eur J Pharmacol. 2011;650(2-3):694-702. https://doi.org/10.1016/j.ejphar.2010.10.060

  11. Xia X, Weng J. Targeting metabolic syndrome: candidate natural agents. J Diabetes. 2010;2(4):243-9. https://doi.org/10.1111/j.1753-0407.2010.00090.x

  12. Mustofa MS, Muchtar D, Panjiasih TS, Royhan A. Pengaruh kedelai (Glycine max (L.) Merril) terhadap kadar glukosa darah dan ekspresi insulin sel B pankreas pada tikus diabetik. YARSI Med J. 2010;18(2):94-103.

  13. Etuk EU. Animals models for studying diabetes mellitus. Agric Biol J N Am. 2010;1(2):130-4.

  14. Prochazkova D, Bousova I, Wilhelmova N. Antioxidant and prooxidant properties of flavonoids. Fisioterapia. 2011;82(4):513-23. https://doi.org/10.1016/j.fitote.2011.01.018

  15. Hiyama E, Hiyama K, Yokoyama T, Shay JW. Immunohistochemical detection of telomerase (hTERT) protein in human cancer tissues and a subset of cells in normal tissues. Neoplasia. 2001;3(1):17-26. https://doi.org/10.1038/sj.neo.7900134

  16. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Scientific World J. 2013;2013:162750. https://doi.org/10.1155/2013/162750

  17. Abd El Latif MA, Mohamed NH, Zaki NL, Abbas MS, Sobhy HM. Effects of Soybean isoflavone on lipid profiles and antioxidant enzyme activity in streptozotocin induced diabetic rats. Global J Pharmacol. 2014;8(3):378-84. https://doi.org/10.5829/idosi.gjp.2014.8.3.1116

  18. Duarte MC, Babeto E, Leite KR, Miyazaki K, Borim AA, Rahal P, et al. Expression of TERT in precancerous gastric lesions compared to gastric cancer. Braz J Med Biol Res. 2011;44(2):100-4. https://doi.org/10.1590/S0100-879X2010007500143

  19. Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KN, Salleh MS, Gurtu S. Effect of glibenclamide alone versus glibenclamide and honey on oxidative stress in pancreas of streptozotocininduced diabetic rats. Intl J Appl Res Nat Prod. 2011;4(2):1-10.

  20. Nasir NFM, Kannan TP, Sulaiman SA, Shamsuddin S, Ahmad A, Stangaciu S. Telomeres and oxidative stress. Br J Med Med Res. 2014;4(1):57-67. https://doi.org/10.9734/BJMMR/2014/5548

  21. Ahmed S, Passos JF, Birket MJ, Beckmann T, Brings S, Peters H, et al. Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress. J Cell Sci. 2008;121(Pt 7):1046-53. https://doi.org/10.1242/jcs.019372

  22. Zisuh AV, Han TQ, Zhan SD. Expression of telomerase & its significance in the diagnosis of pancreatic cancer. Indian J Med Res. 2012;135(1):26-30. https://doi.org/10.4103/0971-5916.93420

Published
2019-10-04
How to Cite
1.
Mustofa MS, Suyatna FD, Sadikin M, Pujianto DA, Royhan A, Suwardji K. Soybean extract increases telomerase reverse transcriptase protein expression in pancreatic β-cells of diabetes mellitus-induced rats. Med J Indones [Internet]. 2019Oct.4 [cited 2024Apr.20];28(3):207-14. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1732
Issue
Vol. 28 No. 3 (2019): September
Section
Basic Medical Research

Copyright (c) 2019 Muhammad Samsul Mustofa, Franciscus D Suyatna, Muhamad Sadikin, Dwi Ari Pujianto, Aan Royhan, Kenconoviati Suwardji

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