Bryophyllum pinnatum leaves ethanol extract inhibit maturation and promote apoptosis of systemic lupus erythematosus BALB/c mice B cells

  • Kusworini Handono Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang
  • Tri W.I. Dantara Biomedical Sciences Master Program, Faculty of Medicine, Universitas Brawijaya, Malang
  • Elvira S. Dewi Nursing Program, Faculty of Medicine, Universitas Brawijaya, Malang
  • Mirza Z. Pratama Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya, Malang
  • Nurdiana Nurdiana Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang
Keywords: B cells, Bryophyllum pinnatum, pristane, SLE
Abstract viewed: 961 times
PDF downloaded: 540 times
HTML downloaded: 8 times
EPUB downloaded: 10 times

Abstract

Background: B cells play a key role in systemic lupus erythematosus (SLE). Targeting B cells as SLE therapy is a plausible approach. This study investigated the potential effects of Bryophyllum pinnatum leaves with ethanol extract in decreasing percentages of maturation, increasing percentages of apoptosis, and decreasing NF-κB p65 expressions of SLE BALB/c mice B cells.

Methods: Culturing B cells from pristane induced SLE BALB/c mice’s spleen will resulted in this in vitro study. B cells were activated by BAFF, LPS, IL-4, and anti-CD40 yielding CD19+ >80%. B cells were cultured by adding those stimulants with and without B. pinnatum leaves (0, 0.02, 0.1, or 0.5 µg/ml) for 72 hours at 37°C. Flow cytometry was performed to determine The Percentages of maturation (CD19+CD38+) and apoptosis (Annexin V+PI+) of B cells.  Further analysis to determine the expressions of transcription factor of maturation and apoptosis of B cells, NF-ĸB p65, were performed using immunocytochemistry. Data were analyzed using SPSS version 22.

Results: Flow cytometry assay showed significant decrease in percentages of maturation of B cells in all doses and significant increase in percentage of apoptosis of B cells in dose 0.5 µg/ml. Immunocytochemistry results showed significant decrease expressions of NF-ĸB p65 in all doses. Percentages of maturation, apoptosis, and expressions of NF-ĸB p65 of B cells were significantly correlated.

Conclusion: This in vitro study revealed that B. pinnatum leaves with ethanol extract decreased the percentages of maturation, increased the percentage of apoptosis, and decreased NF-κB p65 expressions of SLE BALB/c mice B cells significantly.

References

  1. Jacob N, Stohl W. Autoantibody-dependent and autoantibody-independent roles for B cells in systemic lupus erythematosus: past, present, and future. Autoimmunity. 2010; 43(1): 84–97. https://doi.org/10.3109/08916930903374600

  2. Pateinakis P, Pyrpasopoulou A. Targeting the B-cell pathway in lupus nephritis: current evidence and future perspectives. Sci World J. 2013;2013:745239. https://doi.org/10.1155/2013/745239

  3. Leiss H, Niederreiter B, Bandur T, Schwarzecker B, Blüml S, Steiner G, et al. Pristane-induced lupus as a model of human lupus arthritis: evolvement of autoantibodies, internal organ and joint inflammation. Lupus. 2013;22(8):778–92. https://doi.org/10.1177/0961203313492869

  4. Reeves WH, Lee PY, Weinstein JS, Satoh M, Lu L. Induction of autoimmunity by pristane and other naturally occurring hydrocarbons. Trends Immunol. 2009;30(9):455–64. https://doi.org/10.1016/j.it.2009.06.003

  5. Zhuang H, Szeto C, Han S, Yang L, Reeves WH. Animal models of interferon signature positive lupus. Front Immunol. 2015;6(291):1–6. https://doi.org/10.3389/fimmu.2015.00291

  6. Postal M, Costallat LT, Appenzeller S. Biological therapy in systemic lupus erythematosus. Int J Rheumatol. 2012;2012:578641. https://doi.org/10.1155/2012/578641

  7. Anolik JH. B cell biology: implications for treatment of systemic lupus erythematosus. Lupus. 2013;22(4):342–9. https://doi.org/10.1177/0961203312471576

  8. Furie R, Petri M, Zamani O, Cervera R, Wallace DJ, Tegzová D, et al. A phase III, randomized, placebo-controlled study of belimumab, a monoclonal antibody that inhibits B lymphocyte stimulator, in patients with systemic lupus erythematosus. Arthritis Rheum. 2011;63(12):3918–30. https://doi.org/10.1002/art.30613

  9. Hahn BH. Belimumab for systemic lupus erythematosus. N Engl J Med. 2013;368(16):1528–35. https://doi.org/10.1056/NEJMct1207259

  10. Vincent FB, Morand EF, Schneider P, Mackay F. The BAFF/APRIL system in SLE pathogenesis. Nat Rev Rheumatol. 2014;10(6):365–73. https://doi.org/10.1038/nrrheum.2014.33

  11. Fürer K, Simões-Wüst AP, Von Mandach U, Hamburger M, Potterat O. Bryophyllum pinnatum and related species used in anthroposophic medicine: constituents, pharmacological activities, and clinical efficacy. Planta Med. 2016;82(11–12):930–41. https://doi.org/10.1055/s-0042-106727

  12. Oufir M, Seiler C, Gerodetti M, Gerber J, Fürer K, Mennet-von EM, et al. Quantification of bufadienolides in Bryophyllum pinnatum leaves and manufactured products by UHPLC-ESIMS/MS. Planta Med. 2015;81(12–13):1190–7. https://doi.org/10.1055/s-0035-1546126

  13. Anjoo K, Kumar SA. Microscopical and preliminary phytochemical studies on aerial part (leaves and stem) of Bryophyllum Pinnotum Kurz. Phcog J. 2010;2(9):254–9. https://doi.org/10.1016/S0975-3575(10)80113-0

  14. Nwali BU, Okaka ANC, Ibiam UA, Aja PM. Phytochemical composition of Bryophyllum pinnatum leaves. Int J Adv Biol Res. 2012;2(4):614–6.

  15. Zhang X-A, Zhang S, Yin Q, Zhang J. Quercetin induces human colon cancer cells apoptosis by inhibiting the nuclear factor-kappa B pathway. Pharmacogn Mag. 2015;11(42):404–9. https://doi.org/10.4103/0973-1296.153096

  16. Simões-Wüst AP, Jeschke E, Mennet M, Schnelle M, Matthes H, von Mandach U. Prescribing pattern of Bryophyllum preparations among a network of anthroposophic physicians. Forsch Komplementmed. 2012;19(6):293–301. https://doi.org/10.1159/000345841

  17. Gründemann C, Diegel, C, Sauer B, Garcia-Käufer M, Huber R. Immunomodulatory effects of preparations from Anthroposophical Medicine parenteral use. BMC Complement Altern Med. 2015;15:219. https://doi.org/10.1186/s12906-015-0757-6

  18. Czabotar PE, Lessene G, Strasser A, Adams JM. Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy. Nat Rev Mol Cell Biol. 2014;15(1):49–63. https://doi.org/10.1038/nrm3722

  19. Kalim H, Handono K, Khalasa T, Pratama MZ, Dantara TWI, Wulandari AP, et al. Immune modulation effects of curcumin in pristane induced lupus mice. Indian J Rheumatol. 2017;12(2):86–93. https://doi.org/10.4103/injr.injr_95_16

  20. Pawar RD, Goilav B, Xia Y, Zhuang H, Herlitz L, Reeves WH. Serum autoantibodies in pristane induced lupus are regulated by neutrophil gelatinase associated lipocalin. Clin Immunol. 2014;154(1):49–65. https://doi.org/10.1016/j.clim.2014.06.007

  21. Yaniv G, Twig G, Shor DBA, Furer A, Sherer Y, Mozes O, et al. A volcanic explosion of autoantibodies in systemic lupus erythematosus: a diversity of 180 different antibodies found in SLE patients. Autoimmun Rev. 2015;14(1):75–9. https://doi.org/10.1016/j.autrev.2014.10.003

Published
2018-02-14
How to Cite
1.
Handono K, Dantara TW, Dewi ES, Pratama MZ, Nurdiana N. <em>Bryophyllum pinnatum</em&gt; leaves ethanol extract inhibit maturation and promote apoptosis of systemic lupus erythematosus BALB/c mice B cells. Med J Indones [Internet]. 2018Feb.14 [cited 2019Dec.6];26(4):253-60. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1899
Section
Basic Medical Research