Analysis of fatty acids in Ghee and olive oil and their probable causal effect in lipoid pneumonia

  • Zein Mirghani
  • Tayseer Zein
  • Samuel Annoble
  • John Winter
  • Randa Mostafa
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Aim: To analyze and identify the fatty acids found in homemade ghee and in olive oil and compare those to fatty acids found in bronchoalevolar lavage of children with lipoid pneumonia.

Methods: The fatty acids found in homemade fat â€Ghee†and olive oil were analyzed by gas chromatography. Methyl ester derivatives suitable for GC analysis were prepared directly from olive oil or from Ghee using anhydrous methanolic-HCl. Bronchoscopy and bronchoalevolar lavage was performed in eight children aged between 2 and 4 years, all with history of using homemade ghee and/or olive oil in the recumbent position.

Results: The analysis of fatty acids in Ghee and olive oil show similar gas chromatographic pattern as those of bronchoalevolar lavage.

Conclusion: The three fatty acids responsible for the deleterious effects of lipoid pneumonia were identified. Lipoid pneumonia should be one of the differentials diagnosis in children  presenting with respiratory distress. (Med J Indones 2010; 19:252-7)

Keywords: Bronchoalevolar lavage, gas chromatography, Ghee, methyl esters, lipoid pneumonia


  1. Banjar H. Lipoid pneumonia. A Review. Bahrain Med.

  2. Bull. 2003; 25:36-9.

  3. Furuya ME, Cordova M, Figueroa R, Vargas MH, Garcia

  4. RG, Juan RS. Cutoff values of lipid-laden alveolar

  5. macrophages for diagnosing aspiration in infants and

  6. children. Paediatr Pulmonol. 2007, 5:452-7.

  7. Gil A. Polyunsaturated fatty acids and infl ammatory

  8. disease. Biomed Pharmacother. 2002; 56(8):388-96.

  9. Betancourt SL, Martinez JS, Rossi SE, Truong MT, Carrillo

  10. J, Erasmus JJ. Lipoid pneumonia: spectrum of clinical and radiologic manifestations. Am J Roentgenol. 2010; 194(1):103-9.

  11. Siasi SM, Ferreirall AS, Daltrolli PA, Caetano RL, Moreira JS, Santos TQ. Evolution of exogenous lipoid pnuomonia in children: clinical aspects, radiological aspects and role of bronchoalevolar ravage. J Bra Pneumol 2009; 35(9):839-45.

  12. Franquet T, Gimenez A, Roson N, Torrubia S, Sabate J, Perez G. Aspiration Disease, Pitfalls, and Dfferential Diagnosis. Radiographics 2000; 20:673-5.

  13. Al-Kindi H, Abdoani R, EL-Iraqi M, Praseeda I. Lipoid Pemonia Following Aspiration of Ghee (animal fat) in an Omani Infant. Oman Med J. 2008; 23:123-5.

  14. Ohwada A, Yoshioka Y, Shimanuki Y, Mitani K, Kumasaka

  15. T, Dambara T, Fukuchi Y. Exogenous lipoid pneumonia following ingestion of liquid paraffi n. Intern Med. 2002; 41(6):483-6.

  16. Hoffman LR, Elizabeth HY, Kanne JP, Effmann EL, Roland

  17. LG, Cornelius WV. Lipoid pneumonia due to Mexican folk remedies. Arch Pediatr Adolesc Med. 2005; 159:1043-8.

  18. Adhisivam B, Mahadevan S. Olis that spoil the child. Ind J

  19. Paed. 2006; 73(6):88-9.

  20. Kassarjan Y. An infant with respiratory distress. Clin

  21. Paediatr. 2001; 40:507-9.

  22. Rossi SE, Franquet T, Volpacchino M, Gimenez A, Aguilar

  23. G. Tree-in Bud Pattern AT Thin-Section CT of the Lung: Radiologic-Pathologic Overview. RadioGraphics 2005; 25:789-801.

  24. Zanetti G, Marchiori E, Gasparetto T, Escuissato D, Souza

  25. A. Lipoid pnenuomnia in children following mineral oil used in treatment of constipation: high resolution CT fi ndings in 17 patients.Pediatr Radiol. 2007; 37:1135-9.

  26. Khilnani GC, Hadda V. Lipoid pneumonia: an uncommon

  27. entity. Indian J Med Sci. 2009, 63:474-80.

  28. Sias S, Pedro A, Daltro M, Marchiori E, Angela S, Ferreria

  29. M, Regina L, Caetano M, Cleonice S, Silva M, Muller M, Moreira J, Santos T. Clinic and radiological improvement Vol. 19, No. 4, December 2010 Fatty acids and lipoid pneumonia 257 of lipoid pneumonia with multiple bronchalveolar lavages. Pediatr Pulmol. 2009, 44(4):309-15.

  30. Shira B, Haramati L, Vivan R. Radiological and clinical findings in acute and chronic exogenous lipoid pneumonia. J Thoracic Imaging 2003; 8(4):217-24.

  31. Banjar HH. Areview of 151 cases of pediatric noncystic

  32. fi brosis bronchioectasis in tertiary care center. Ann Throc

  33. Med. 2007; 2:3-8.

  34. Bobbak V, Timothy L. Complementary medicines and

  35. pulmonary toxicities: what a chest physician should know.

  36. Clin Pulmo Med. 2007; 14(6):338-45.

  37. Christie W. W. Lipid analysis, London: Pergamon

  38. Press;1973. p88

  39. Choong Y, Lin H. Gas chromatographic determination of

  40. synthetic antioxidants in edible fats and oils – a simple methylation method. J Food and Drug Analysis 2001; 1(9):20-6

  41. Mirghani Z, Morad NA, Annobil SH, Adzaku FK, Kameswaran M. Effects of different types of oil aspiration in albino rats and its correlation with humans. Biomed Res. 2006; 17: 7-11.

  42. Harizi H, Nobert G. 5-Lipoxygenase pathway: Dendritic

  43. cells, and adaptive immunity. J Biomed Biotechnol. 2004; 2:99-105.

  44. N, Triaspolitica. "Kanker Payudara: Informasi, Penyebab, Gejala, Stadium Dan Pengobatan." Mau Nanya Dong Dok. N.p, 28 June 2017. Web. 30 June 2017.<>.

  45. airway hyperresponsiveness after ozone inhalation. Am J Respir Crit Care Med. 2010; 181(7):666-75.

  46. Mylonaki E, Voutas V, Antoniou D, Papakosta D, Kontakiotis

  47. T, Skordalaki A, Vafi adis E, Christaki P. Hydrocarbon

  48. pneumonitis following liquid paraffi n aspiration during a

  49. fi re-eating performance: a case report. J Med Case Reports

  50. ; 19(2): 214-5.

How to Cite
Mirghani Z, Zein T, Annoble S, Winter J, Mostafa R. Analysis of fatty acids in Ghee and olive oil and their probable causal effect in lipoid pneumonia. Med J Indones [Internet]. 2010Nov.1 [cited 2020Sep.28];19(4):252-7. Available from:
Clinical Research