Colorectal cancer among young native Indonesians: A clinicopathological and molecular assessment on microsatellite instability

Aru W. Sudoyo; Bethy Hernowo; Ening Krisnuhoni; Ary H. Reksodiputro; Daldiyono Hardjodisastro

doi:10.13181/mji.v19i4.411

Authors

Aru W. Sudoyo
Bethy Hernowo
Ening Krisnuhoni
Ary H. Reksodiputro
Daldiyono Hardjodisastro

DOI:

https://doi.org/10.13181/mji.v19i4.411

Abstract

Aim: To obtain clinicopathological characteristics of colorectal cancer among young native Indonesians and to assess MLH1, MSH2, and SMAD4 protein expressions, comparing them with a matched population of colorectal cancer patients aged 60 years old and older.

Methods: Medical records of colorectal cancer patients aged 40 years or younger and 60 years or older from several hospitals in three Indonesian cities â Jakarta, Makassar, and Bandung - were reviewed. The ânativeâ ethnic groups were selected from those originating from Java, Makassar (South Celebes),Â Minangkabau (West Sumatra). Ethnicity of 121 colorectalÂ carcinoma patients was confirmed by fulfilling requirements in a questionnaire. Tumor specimens of those patients underwent evaluation for histopathology, tumor grading as well asÂ immunohistochemical analysis to assess MLH1, MSH2 protein expressions to detect microsatellite instability mutation pathway and SMAD4 protein expression to reconfirm that the specimens were not microsatellite instability origin.

Results: There were 121 colorectal carcinoma cases of Sundanese, Javanese, Macassarese and Minangkabau ethnic group. This study indicated that colorectal cancer has statistically different grade (p = 0.001) between the young and the older patients. Immunohistochemical staining for MSH2 protein and MLH1 were done for 92 and 97 specimens respectively. There was no significant difference between the expressions of MLH1 and MSH2 on tumor grading, indicated there was no correlation between microsatellite instability and tumor grading in this study.

Conclusion: Colorectal cancer in young native Indonesian patients (40 years old or less) was not different in clinicopathological characteristics compared to older patients (60 years old or more) in similar ethnic groups. There was also no difference in MSH2 and MLH1 protein expressions, important indicators of microsatellite instability and. (Med J Indones 2010; 19:245-51)

Keywords: colorectal cancer, microsatellite instability, MLH1, MSH2, native Indonesian, SMAD4

Downloads

Download data is not yet available.

References

Banjar H. Lipoid pneumonia. A Review. Bahrain Med. Bull. 2003; 25:36-9.

Furuya ME, Cordova M, Figueroa R, Vargas MH, Garcia RG, Juan RS. Cutoff values of lipid-laden alveolar acrophages for diagnosing aspiration in infants and children. Paediatr Pulmonol. 2007, 5:452-7.

Gil A. Polyunsaturated fatty acids and infl ammatory disease. Biomed Pharmacother. 2002; 56(8):388-96.

Betancourt SL, Martinez JS, Rossi SE, Truong MT, Carrillo J, Erasmus JJ. Lipoid pneumonia: spectrum of clinical and radiologic manifestations. Am J Roentgenol. 2010; 194(1):103-9.

Siasi SM, Ferreirall AS, Daltrolli PA, Caetano RL, MoreiraJS, Santos TQ. Evolution of exogenous lipoid pnuomonia in children: clinical aspects, radiological aspects and role of bronchoalevolar ravage. J Bra Pneumol 2009; 35(9):839-45.

Franquet T, Gimenez A, Roson N, Torrubia S, Sabate J, Perez G. Aspiration Disease, Pitfalls, and Dfferential Diagnosis. Radiographics 2000; 20:673-5.

Al-Kindi H, Abdoani R, EL-Iraqi M, Praseeda I. Lipoid Pemonia Following Aspiration of Ghee (animal fat) in an Omani Infant. Oman Med J. 2008; 23:123-5.

Ohwada A, Yoshioka Y, Shimanuki Y, Mitani K, Kumasaka T, Dambara T, Fukuchi Y. Exogenous lipoid pneumonia following ingestion of liquid paraffi n. Intern Med. 2002; 41(6):483-6.

Hoffman LR, Elizabeth HY, Kanne JP, Effmann EL, Roland LG, Cornelius WV. Lipoid pneumonia due to Mexican folkremedies. Arch Pediatr Adolesc Med. 2005; 159:1043-8.

Adhisivam B, Mahadevan S. Olis that spoil the child. Ind J Paed. 2006; 73(6):88-9.

Kassarjan Y. An infant with respiratory distress. Clin paediatr. 2001; 40:507-9.

Rossi SE, Franquet T, Volpacchino M, Gimenez A, Aguilar G. Tree-in Bud Pattern AT Thin-Section CT of the Lung: Radiologic-Pathologic Overview. RadioGraphics 2005;25:789-801.

Zanetti G, Marchiori E, Gasparetto T, Escuissato D, Souza A. Lipoid pnenuomnia in children following mineral oil used in treatment of constipation: high resolution CT fi ndings in 17 patients.Pediatr Radiol. 2007; 37:1135-9.

Khilnani GC, Hadda V. Lipoid pneumonia: an uncommon entity. Indian J Med Sci. 2009, 63:474-80.

Sias S, Pedro A, Daltro M, Marchiori E, Angela S, Ferreria M, Regina L, Caetano M, Cleonice S, Silva M, Muller M, Moreira J, Santos T. Clinic and radiological improvement Vol. 19, No. 4, December 2010 Fatty acids and lipoid pneumonia 257 of lipoid pneumonia with multiple bronchalveolar lavages. Pediatr Pulmol. 2009, 44(4):309-15.

Shira B, Haramati L, Vivan R. Radiological and clinical fi ndings in acute and chronic exogenous lipoid pneumonia. J Thoracic Imaging 2003; 8(4):217-24.

Banjar HH. Areview of 151 cases of pediatric noncystic fi brosis bronchioectasis in tertiary care center. Ann Throc Med. 2007; 2:3-8.

Bobbak V, Timothy L. Complementary medicines and pulmonary toxicities: what a chest physician should know. Clin Pulmo Med. 2007; 14(6):338-45

Christie W. W. Lipid analysis, London: Pergamon Press;1973. p88

Choong Y, Lin H. Gas chromatographic determination of synthetic antioxidants in edible fats and oils - a simple methylation method. J Food and Drug Analysis 2001;1(9):20-6

Mirghani Z, Morad NA, Annobil SH, Adzaku FK, Kameswaran M. Effects of different types of oil aspiration in albino rats and its correlation with humans. Biomed Res. 2006; 17: 7-11.

Harizi H, Nobert G. 5-Lipoxygenase pathway: Dendritic cells, and adaptive immunity. J Biomed Biotechnol. 2004;2:99-105.

Garantziotis S, Li Z, Potts EN, Lindsey JY, Stober VP, Polosukhin VV, Blackwell TS, Schwartz DA, Foster WM, Hollingsworth JW. TLR4 is necessary for hyaluronanmediated airway hyperresponsiveness after ozone inhalation. Am J Respir Crit Care Med. 2010; 181(7):666-75.

Mylonaki E, Voutas V, Antoniou D, Papakosta D, Kontakiotis T, Skordalaki A, Vafi adis E, Christaki P. Hydrocarbon pneumonitis following liquid paraffi n aspiration during a fi re-eating performance: a case report. J Med Case Reports 2008; 19(2): 214-5.