Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

  • Seruni K.U. Freisleben
  • Jenny Hidayat
  • Hans-Joachim Freisleben
  • Soehardjo Poertadji
  • Budi Kurniawan
  • Na P. Bo
  • Sri Handayani
  • Pustika A. Wahidiyat
  • Rondang R. Soegianto
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Abstract

Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage.

Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10 controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status were measured. Isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (EPR) spectroscopy using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA.

Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. Vitamin E, group C: 21.8 vs T: 6.2 μmol/L) and reactive thiols (C: 144 vs. T: 61 μmol/L) were considerably lower in transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 μmol/L) and of tissue cell damage, i.e., high transaminases plasma levels. Non-transfused thalassemia patients have slight signs of oxidative stress, but no significant indication of cell damage. Erythrocyte membrane parameters from EPR spectroscopy differ considerably between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized.

Conclusion: In tranfusion-dependent thalassemic patients, plasma lipid pattern and oxidative stress are associated with structural damage of isolated erythrocyte membranes as measured by EPR spectroscopy with lipid and proteinthiol spin labels. (Med J Indones 2011; 20:178-84)

Keywords: electron paramagnetic resonance spectroscopy, erythrocyte membrane, lipoproteins, oxidative stress, thalassemia, plasma lipids.

Published
2011-08-01
How to Cite
1.
Freisleben SK, Hidayat J, Freisleben H-J, Poertadji S, Kurniawan B, Bo NP, Handayani S, Wahidiyat PA, Soegianto RR. Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta. Med J Indones [Internet]. 2011Aug.1 [cited 2019Nov.12];20(3):178-84. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/448
Section
Basic Medical Research

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