Plasma lipid pattern and red cell membrane structure in Î²-thalassemia patients in Jakarta
Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive pictureÂ of iron overload, oxidative stress, and cell damage.
Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10Â controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status wereÂ measured. Isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (EPR) spectroscopyÂ using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA.
Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls.Â Vitamin E, group C: 21.8 vs T: 6.2 Î¼mol/L) and reactive thiols (C: 144 vs. T: 61 Î¼mol/L) were considerably lower inÂ transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 Î¼mol/L) and of tissue cell damage,Â i.e., high transaminases plasma levels. Non-transfused thalassemia patients have slight signs of oxidative stress, but noÂ significant indication of cell damage. Erythrocyte membrane parameters from EPR spectroscopy differ considerablyÂ between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarityÂ and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized.
Conclusion: In tranfusion-dependent thalassemic patients, plasma lipid pattern and oxidative stress are associatedÂ with structural damage of isolated erythrocyte membranes as measured by EPR spectroscopy with lipid and proteinthiolÂ spin labels. (Med J Indones 2011; 20:178-84)
Keywords: electron paramagnetic resonance spectroscopy, erythrocyte membrane, lipoproteins, oxidative stress,Â thalassemia, plasma lipids.
Copyright (c) 2011 Seruni K.U. Freisleben, Jenny Hidayat, Hans-Joachim Freisleben, Soehardjo Poertadji, Budi Kurniawan, Na P. Bo, Sri Handayani, Pustika A. Wahidiyat, Rondang R. Soegianto
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with Medical Journal of Indonesia agree to the following terms:
- Authors retain copyright and grant Medical Journal of Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial License that allows others to remix, adapt, build upon the work non-commercially with an acknowledgment of the work’s authorship and initial publication in Medical Journal of Indonesia.
- Authors are permitted to copy and redistribute the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Medical Journal of Indonesia.