The validity of rapid test to detect prostate-specific antigen (PSA) in seminal fluid

  • Henky Henky
  • Yuli Budiningsih
  • Wibisana Widiatmaka
Keywords: PSA, rapid test, sexual assault
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Background: This study was conducted to determine whether the rapid test device can be used to detect PSA in seminal fluid specifically, for solving sexual assault cases.

Methods: A cross sectional study has been conducted. A total of 45 samples were taken consecutively. Semen was diluted in serially up to 1:5.000.000 and male urin up to 1:200 using distilled water, whereas female urin was not diluted. Samples were analyzed using rapid test PSA.

Results: The proportion of positive results of PSA in seminal fluid, male urin and female urin respectively was 100%, 6.67%, and 0%. Statistically, these differences are highly significant. The analysis revealed that the PSA rapid test device was 100% sensitive and 96.67% specific to detect seminal fluid. The test also have PPV 93.75%, NPV 100%, LR(+) 33.33, LR(-) 0, and AUC 0.983.

Conclusion: PSA Rapid Test is very specific and sensitive to detect PSA in seminal fluid. Therefore, this device is suggested for forensic use in sexual assault cases. (Med J Indones 2011; 20:278-82)

How to Cite
Henky H, Budiningsih Y, Widiatmaka W. The validity of rapid test to detect prostate-specific antigen (PSA) in seminal fluid. Med J Indones [Internet]. 2011Nov.1 [cited 2024Apr.19];20(4):278-82. Available from:
Clinical Research