Multi-hole spherical CT scan method to characterize large quantities of bones in rats

  • Neng Nenden Mulyaningsih Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Department of Physics Education, Faculty of Mathematics and Natural Sciences, Universitas Indraprasta PGRI, Jakarta, Indonesia
  • Ariadne Lakshmidevi Juwono Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia
  • Djarwani Soeharso Soejoko Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia
  • Dewi Apri Astuti Department of Nutrition and Feed Technology, Faculty of Animal Sciences, Institut Pertanian Bogor, Bogor, Indonesia
Keywords: bone quality, CT scan, multi-hole spherical model, osteoporosis, ovariectomy
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BACKGROUND New therapeutic options are often explored in in vivo studies using animals like rats. Since rats are small, it is difficult to examine them in a computed tomography (CT) scan. This study aimed to introduce a multi-hole spherical model CT scan method as a new, fast, economical, and reliable method to characterize large quantities of rat bones at once in estimating the timing of osteoporosis in ovariectomized white rats.

METHODS 50 female white rats (12 weeks old) were treated as the control group, and 40 rats of the same age were ovariectomized to establish the osteoporosis model. Sham rats were sacrificed at 13, 15, 17, 19, and 21 weeks old, while the ovariectomized rats were sacrificed at 15, 17, 19, and 21 weeks old. Afterward, tibia bones were removed, placed in the multi-hole spherical model, and characterized using a CT scan. Their characteristics were compared using a scanning electron microscope (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD).

RESULTS The Hounsfield unit scores resulted from the multi-hole spherical model CT scan method of tibia bones of rats were consistent with the percentage of the osteocyte cavities, canalicular diameters, and crystal size. The multi-hole spherical model CT scan method could produce 50 times more data than the SEM, TEM, or XRD.

CONCLUSIONS Multi-hole spherical model CT scan was considered good and reliable in assessing bone quality parameters in rat samples simultaneously.


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How to Cite
Mulyaningsih NN, Juwono AL, Soejoko DS, Astuti DA. Multi-hole spherical CT scan method to characterize large quantities of bones in rats. Med J Indones [Internet]. 2021Oct.1 [cited 2024Apr.19];30(3):182-90. Available from:
Basic Medical Research

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