Blood lactate level in Wistar rats after four and twelve week intermittent aerobic training

  • Dewi N. Sari Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Sutjahjo Endardjo Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Dewi I.S. Santoso Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Keywords: Blood lactate, intermittent aerobic training, Wistar rat


Background: Aerobic training can be done not only continuously, but also intermittently. Intermittent aerobic training aimed to get blood lactate level lower than continuous aerobic training. Blood lactate concentration in one of the various factors that determine training performance. However, until recently, little studies about intermittent aerobic training and blood lactate levels have been done. Therefore, this study aimed to measure blood lactate levels in Wistar rats after 4 and 12 weeks of intermittent aerobic training.

Methods: 16 Wistar rats were divided into two groups, control and aerobic group. Every group was divided into two subgroups, 4-week and 12-week subgroup. Aerobic group performed training using T-6000 treadmill with a speed of 20 m/minute for 20 minutes, with resting period for 90 seconds every 5 minute. Measurements of lactate level was done with L-lactate (PAP) Randox kit (LC2389).

Results: Blood lactate level in the 4-week aerobic group was 2.11 mmol/L, while that of the 4-week control group was 1.82 mmol/L (p > 0.05). Meanwhile, lactate level in 12-week aerobic group was 1.71 mmol/L (p < 0.05), and significantly lower than in 12-week control group, which was 3.03 mmol/L.

Conclusion: This study showed that lactate level after 12-week intermittent aerobic training was the lowest compared to 4-week intermittent aerobic and 12-week control group.


Gladden LB. Lactate metabolism: a new paradigm for the third millenium. J physiol. 2004; 558 (1): 5-30.

Wilmore JH,Costill DL, Kenney WL. Fuel for exercising muscle: metabolism and hormonal control. In: Wilmore JH,Costill DL, Kenney WL, editors. Physiology of sport and exercise. 4th ed. Champaign Illinois: Human Kinetics;2008, p.48-59.

Svedahl K, Macintosh BR. Anaerobic Threshold: the concept and methods of measurement. Can J Appl Physiol. 2003;28(2):299-323.

Plowman SA, Smith DL. Exercise physiology for health, fitness and performance. 3rd ed. Philadelphia: Lippincott Williams and Wilkins; 2011.

Manchado FB, Gobatto CA, Contarteze RV, Papoti M, De Mello MAR. Maximal lactate steady state in running rats. JEPonline. 2005;8(4):29-35.

Høydal MA, Wisløff U, Kemi OJ, Ellingsen O. Running speed and maximal oxygen uptake in rats and mice : pratical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007;14(6);753-60.

Wang Y, Wisloff U, Kemi O.J. Animal models in the study of exercise induced cardiac hypertrophy. Physiol Res. 2010;59(5): 633-44.

Doggrell SA, Brown L. Rat model of hypertention, cardiac hypertrophy and failure. Cardiovasc Res. 1998;39(1):89-105.

Daussin FN, Zoll J, Dufour SP, et al. Effect of interval versus continuous training on cardiorespiratory and mitochondrial function: relationship to aerobic performance improvement in sedentary subjects. Am J Physiol Regul Integr Comp Physiol. 2008;295(1): R264-72.

Fenning A, Harrison G, Dwyer D, Meyer RR, Brown L. Cardiac adaption to endurance exercise in rats. Molecular and cellular biochemistry. 2003;251:51-9.

Smith C, Marks AD, Lieberman M. Markâs basics medical biochemistry. 2nd ed. Baltimore: Lippincott Williams and Wilkins; 2006.

Donovan CM, Brooks GA. Endurance trainnig affects lactate clearance, not lactate production. Am Journal Physiol Endocrinol Metabolism. 1983; 244(1):83-9.

Díaz-Herrera P, Torres A, Morcuende JA, García-Castellano JM, Calbet JA, Sarrat R. Effect of endurance running on cardiac and skeletal muscle in rats. Histol histopathol. 2001;16(1):29-35.

Bonen A. Lactate transporters (MCT proteins) in heart and skeletal muscles. Medical Science sports exercise. 2000;32(4):778-89.

Siu PM, Donley DA, Bryner RW, Alway SE. Citrate synthase expression and enzyme activity after endurance training in cardiac and skeletal muscles. J Appl physiol. 2003;94(2):555-60.

Gharbi A, Chamari K, Kallel A, Ahmaidi S, Tabka Z, AbdelkarimZ. Lactate kinetics after intermittent and continous exercise training. JSSM. 2008;7:279-85.

How to Cite
Sari DN, Endardjo S, Santoso DI. Blood lactate level in Wistar rats after four and twelve week intermittent aerobic training. Med J Indones [Internet]. 2013Aug.22 [cited 2024Feb.21];22(3):141-5. Available from:
Basic Medical Research