Influence of 17β-estradiol treatment on the expression of NF-κB in complete hydatidiform mole culture
Abstract
Background: Genetic evidence has established a role of nuclear factor kappa B (NF-κB ) signaling in oncogenesis. However, activity of NF-κB in complete hydatidiform mole (CHM) cell culture under 17β-estradiol (E2) treatment is not yet known. Recently, a positive cross-talk between estrogen receptor (ER) and NF-κB to promote survival and progress of cancer cells to a more aggressive phenotype was established. In the present study, we examined the influence of E2 treatment on the NF-κB expression in CHMâs culture.
Methods: This experimental study measured the expression of NF-κB in CHM culture treated with E2: 10, 100, 300, 600, and 1000 pg/mL and without E2. Imunohistochemistry staining was used to assess the expression of NF-κB. Microphotographs were taken using 400x magnification. Adobe photoshop CS2 was used to assess the NF-κB expression in cell nucleus. The lower the color intensity of cell RGBbv, is the higher the expression of NF-κB in cells. ANOVA test was performed to compare the expression of NF-κB.
Results: NF-κB expression as indicated by color intensity in control group was 114.84 ± 9.02. NF-κB expression in E2 treatment groups were respectively: E2 10 pg/mL: 106.30 ± 13.95; E2 100 pg/mL: 82.47 ± 4.72; E2 300 pg/mL: 82.24 ± 2.67; E2 600 pg/mL: 69.05 ± 6.47; E2 1000 pg/mL: 68.49 ± 2.37. There was progressive decline in color intensity of cells with E2 treatment indicating the increase expression of NF-κB. Significant differences with the control group occurred in doses of E2 100, 300, 600, dan 1000 pg/mL.
Conclusion: Treatment of CHM trophoblast culture with escalating doses of E2 was associated with the increase of NF-κB expression in a dose dependent manner. (Med J Indones. 2013;22:197-201. doi: 10.13181/mji.v22i4.599)
References
Martaadisoebrata D. Pola Epidemiologi Penyakit Trofoblas dalam Kaitannya dengan Prognosis dan Cara Penanggulangannya. Bandung: RSHS/FKUP - Divisi Obstetri Ginekologi; 2002. Indonesian.
Sastradinata I. Problematik Penyakit Trofoblas di Indonesia. Palembang: Universitas Sriwijaya - Divisi Obstetri dan Ginekologi; 2009. Indonesian
Yager JD, Davidson NE. Estrogen carcinogenesis in breast cancer. N Engl J Med. 2006;354(3):270-82. http://dx.doi.org/10.1056/NEJMra050776
Dolcet X, Llobet D, Pallares J, Mathias-Guiu X. NF-κB in development and progression of human Cancer. Virchows Arch. 2005;446(5):475-82. http://dx.doi.org/10.1007/s00428-005-1264-9
Cogswell, P.C, Guttridge, D.C, Funkhouser W, Baldwin Jr A. Selective activation of NF-kappa B subunits in human breast cancer: potential roles for NF-kappa B2/p52 and for Bcl-3. Oncogene. 2000;19(9):1123-31. http://dx.doi.org/10.1038/sj.onc.1203412
Feldman I, Feldman GM, Mobarak C, Dunkelberg JC, Leslie KK. Identification of proteins within the nuclear factor-kappa B transcriptional complex including estrogen receptor-alpha. Am J Obstet Gynecol. 2007;196(4);394.e1-394.e13. http://dx.doi.org/10.1016/j.ajog.2006.12.033
Frasor J, Weaver A, Pradhan M, Dai Y, Miller LD, Lin CY, et al. Positive cross-talk between estrogen receptor and NF-Kappa B in breast cancer. Cancer Res. 2009;69(23):8918-25. http://dx.doi.org/10.1158/0008-5472.CAN-09-2608
Osipo C, Gajdos C, Liu H, Chen B, Jordan VC. Paradoxical action of fulvestrant in estradiol-induced regression of tamoxifen-stimulated breast cancer. J Natl Cancer Inst. 2003;95(21):1597-608. http://dx.doi.org/10.1093/jnci/djg079
King AE, Collins F, Klonisch T, Sallenave JM, Critchley HO, Saunders PT. An additive interaction between the NFkappaB and estrogen receptor signalling pathways in human endometrial epithelial cells. Hum Reprod. 2010;25(2):510-8. http://dx.doi.org/10.1093/humrep/dep421
Nalbandian G, Kovats S. Estrogen, Immunity & Autoimmune Disease. Curr Med Chem–Immun, Endoc & Metab Agents Journal. 2005;5(1):85-91. http://dx.doi.org/10.2174/1568013053005418
Kumar S, Lata K, Mukhopadhyay S, Mukherjee TK. Role of estrogen receptors in pro-oxidative and anti-oxidative actions of estrogens: A perspective. Biochim Biophys Acta. 2010;1800(10):1127-35. http://dx.doi.org/10.1016/j.bbagen.2010.04.011
Stoica GE, Franke TF, Wellstein A, Czubayko F, List HJ, Reiter R, et al. Estradiol rapidly activates Akt via the ErbB2 signaling pathway. Mol Endocrinol. 2007;17(5):818-30. http://dx.doi.org/10.1210/me.2002-0330
Copyright (c) 2013 Tatit Nurseta, Yahya Irwanto, Rahmi Utami
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with Medical Journal of Indonesia agree to the following terms:
- Authors retain copyright and grant Medical Journal of Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial License that allows others to remix, adapt, build upon the work non-commercially with an acknowledgment of the work’s authorship and initial publication in Medical Journal of Indonesia.
- Authors are permitted to copy and redistribute the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Medical Journal of Indonesia.