Diversity of <em>Spa</em> gene between methicillin-resistant and methicillin-sensitive <em>Staphylococcus aureus</em> bacteria in a tertiary referral hospital, Indonesia

  • Sri Amelia Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0001-5864-5926
  • R. Lia Kusumawati Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0002-0459-1026
  • Ridwan Balatif Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0002-6638-3900
  • Tryna Tania Whole Genome Sequence Laboratory, Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Lavarina Winda Biomolecular Laboratory, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Nadya Adlin Syamira Department of Biotechnology, Faculty of Science and Mathematics, Universitas Diponegoro, Semarang, Indonesia
Keywords: bacterial typing techniques, Staphylococcus aureus, tertiary referral hospital

Abstract

BACKGROUND Spa typing is an effective and fast technique to identify the prevalence and spread of Staphylococcus aureus strains based on their spa gene profiles. The distribution of spa types will contribute to control the spread of S. aureus. Little is known regarding the spa types of S. aureus in Indonesia. This study aimed to investigate the diversity of spa gene among S. aureus carriage isolates in North Sumatra Province, Indonesia.

METHODS 79 S. aureus isolates consisting of 39 methicillin-resistant S. aureus (MRSA) and 40 methicillin-susceptible S. aureus (MSSA) carriage isolates were identified by VITEK2 Compact (Biomerieux, Indonesia) to detect mecA gene. All samples underwent spa typing and sequencing.

RESULTS Spa gene was detected among 31/39 (79%) of the MRSA isolates and 24/40 (60%) of the MSSA isolates. Most spa typing genes were identified between 350 and 400 base pair (bp). t258 and t852 were the most prevalence spa types among MRSA and MSSA isolates, respectively.

CONCLUSIONS Many MRSA and MSSA isolates encoded spa gene. The most genes detected were t258 and t852, identified in Germany and Portugal, respectively; while t18977 was initially identified in Malaysia. This indicated a global spread of MRSA according to spa typing.

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Published
2023-08-25
How to Cite
1.
Amelia S, Kusumawati RL, Balatif R, Tania T, Winda L, Syamira NA. Diversity of <em>Spa</em> gene between methicillin-resistant and methicillin-sensitive <em>Staphylococcus aureus</em&gt; bacteria in a tertiary referral hospital, Indonesia. Med J Indones [Internet]. 2023Aug.25 [cited 2024Feb.21];32(2):75-9. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/6862
Section
Basic Medical Research