The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model

Authors

  • Kamalia Layal Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta
  • Ika S. Perdhana Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta
  • Melva Louisa Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta
  • Ari Estuningtyas Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta
  • Vivian Soetikno Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta

DOI:

https://doi.org/10.13181/mji.v26i3.1462

Keywords:

chronic kidney disease, nephrectomy, Nrf2, oxidative stress, quercetin

Abstract

Background: Oxidative stress may play a role in the pathogenesis of (CKD), Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor involved in cell defense mechanism against oxidative stress. In this study, we examined the effect of quercetin, a polyphenplic antioxidant anti fibrosis compund in fruits and vegetables, on the 5/6 nephrectomy-induced CKD progression model rats through modulation of Nrf2 expression.

Methods: Male Sprague-Dawley rats were randomly divided into normal control group (C), untreated 5/6 nephrectomy (Nx), quercetin-treated 5/6 nephrectomy (100 mg/kgBW/day orally) (NxQ), and captopril-treated 5/6 nephrectomy (10 mg/kgBW/day orally) (NxK) for 8 weeks. At the end of study, all animals were sacrified. Urine, blood, and kidney tissues were taken for examination of proteinuria, plasma creatinine, urea, malondialdehyde (MDA), glutathione peroxidase (GPx) activity, Nrf2, Keap1, heme oxygenase-1 (HO-1) expressions, and renal fibrosis.

Results: Quercetin administration did not affect the level of protein in urine, plasma creatinine, and urea. However, it tended to reduce the level of MDA, increase GPx activity, Nrf2, Keap1, and HO-1 expression as well as the degree of fibrosis.

Conclusion: In 5/6 nephrectomized rats, quercetin tended to ameliorate the level of MDA, GPx activity, Nrf2, Keap1, and HO-1 expression. In addition, quercetin tended to decrease the degree of fibrosis in the remnant kidney.

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Author Biography

Vivian Soetikno, Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta

SCOPUS h-index: 13

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Published

2017-11-27

How to Cite

1.
Layal K, Perdhana IS, Louisa M, Estuningtyas A, Soetikno V. The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model. Med J Indones [Internet]. 2017Nov.27 [cited 2024Dec.11];26(3):169-77. Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/1462

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Section

Basic Medical Research
Abstract viewed = 2765 times

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