The effect of moderate-intensity acute aerobic exercise duration on the percentage of circulating CD31+ cells in lymphocyte population

  • Mariani Santosa Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Ermita I.I. Ilyas Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Radiana D. Antarianto Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
Keywords: CD31 peripheral blood mononuclear cells, circulating endothelial progenitor cells, endothelial regeneration, exercise
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Abstract

Background: The increasing number of circulating CD31+ endothelial progenitor cells is one of the important factors for maintaining vascular homeostasis. Exercise will effectively increase the number of circulating CD31+ endothelial progenitor cells. This study aims to determine the effect of moderate-intensity acute aerobic exercise duration on the percentage of circulating CD31+ cells in untrained healthy young adult subjects.

Methods: This study was an experimental study. Untrained healthy volunteers (n=20) performed ergocycle at moderate-intensity (64–74% maximum heart rate) for 10 minutes or 30 minutes. Immediately before and 10 minutes after exercise, venous blood samples were drawn. The percentage of CD31+ cells in peripheral blood was analyzed using flow cytometry. Data was statistically analyzed using student t-test.

Results: There were no significant differences in the mean percentage of circulating CD31+ cells before and after exercise for 10 minutes and 30 minutes (p>0.05). However, there was a different trend in the percentage of circulating CD31+ cells after exercise for 10 minutes and 30 minutes. In the 10 minutes duration, 50% of subjects showed increase. Whereas in the 30 minutes duration, 80% of subjects showed increase.

Conclusion: The percentage of circulating CD31+ cells before and after exercise for 10 minutes was not different compared to 30 minutes. However, data analysis shows that majority of subjects (80%) had increased in the percentage of circulating CD31+ cells after 30 minutes exercise.

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References

  1. Institute of Medicine (US) Committee on Preventing the Global Epidemic of Cardiovascular Disease. Promoting cardiovascular health in the developing world: a critical challenge to achive global health. Fuster V, Kelly BB, editors. Washington (DC): National Academies Press (US); 2010. p. 49.

  2. World Health Organization. Preventing chronic disease: a vital investment. Geneva: World Health Organization; 2005. p. 15.

  3. Rajendran P, Rengarajan T, Thangavel J, Nishigaki Y, Sakthisekaran D, Sethi G, et al. The vascular endothelium and human diseases. Int J Biol Sci. 2013;9(10):1057–69. http://dx.doi.org/10.7150/ijbs.7502

  4. Deanfield JE, Halcox JP, Rabelink TJ. Endothelial function and dysfunction: testing and clinical relevance. Circulation. 2007;115(10):1285–95. http://dx.doi.org/10.1161/CIRCULATIONAHA.106.652859

  5. De Biase C, De Rosa R, Luciano R, De Luca S, Capuano E, Trimarco B, et al. Effects of physical activity on endothelial progenitor cells (EPCs). Front Physiol. 2013;4:414. http://dx.doi.org/10.3389/fphys.2013.00414

  6. Timmermans F, Plum J, Yöder MC, Ingram DA, Vandekerckhove B, Case J. Endothelial progenitor cells: identity defined?. J Cell Mol Med. 2009;13(1):87–102. http://dx.doi.org/10.1111/j.1582-4934.2008.00598.x

  7. Kim H, Cho HJ, Kim SW, Liu B, Choi YJ, Lee J, et al. CD31+ cells represent highly angiogenic and vasculogenic cells in bone marrow: novel role of nonendothelial CD31+ cells in neovascularization and their therapeutic effects on ischemic vascular disease. Circ Res. 2010;10(5):602–14. http://dx.doi.org/10.1161/CIRCRESAHA.110.218396

  8. Koutroumpi M, Dimopoulos S, Psarra K, Kyprianou T, Nanas S. Circulating endothelial and progenitor cells: evidence from acute and long term exercise effects. World J Cardiol. 2012;4(12):312–26. http://dx.doi.org/10.4330/wjc.v4.i12.312

  9. Siddique A, Shantsila E, Lip GYH, Varma C. Endothelial progenitor cells: what use for the cardiologist?. J Angiogenes Res. 2010;2(6):1–13. http://dx.doi.org/10.1186/2040-2384-2-6

  10. American College of Sports Medicine. ACSM's guidelines for exercise testing and prescription. 8th ed. Philadelphia: Lippincott Wiliams & Wilkins; 2010. p. 7–76.

  11. Silva JF, Rocha NG, Nóbrega AC. Mobilization of endothelial progenitor cells with exercise in healthy individuals: a systematic review. Arq Bras Cardiol. 2012;98(2):182–91. http://dx.doi.org/10.1161/hc2301.092122

  12. Sharkey BJ. Physiology of fitness. 3rd ed. USA: Human Kinetics Publishers; 1990. p. 19.

  13. Hristov M, Erl W, Weber PC. Endothelial progenitor cells: mobilization, differentiation, and homing. Arterioscler Thromb Vasc Biol. 2003;23(7):1185–9. http://dx.doi.org/10.1161/01.ATV.0000073832.49290.B5

  14. Laufs U, Urhausen A, Werner N, Scharhag J, Heitz A, Kissner G, et al. Running exercise of different duration and intensity: effect on endothelial progenitor cells in healthy subjects. Eur J Cardiovasc Prev Rehabil. 2005;12(4):407–14. http://dx.doi.org/10.1097/01.hjr.0000174823.87269.2e

  15. Cubbon RM, Murgatroyd SR, Ferguson C, Bowen TS, Rakobowchuk M, Baliga V, et al. Human exercise-induced circulating progenitor cell mobilization is nitric oxide-dependent and is blunted in South Asian men. Arterioscler Thromb Vasc Biol. 2010;30(4):878–84. http://dx.doi.org/10.1161/ATVBAHA.109.201012

  16. Swain DP, Abernathy KS, Smith CS, Lee SJ, Bunn SA. Target heart rates for the development of cardiorespiratory fitness. Med Sci Sports Exerc. 1994;26:112–6. http://dx.doi.org/10.1249/00005768-199401000-00019

  17. Kojda G, Hambrecht R. Molecular mechanisms of vascular adaptations to exercise. Physical activity as an effective antioxidant therapy?. Cardiovasc Res. 2005;67(2):187–97. http://dx.doi.org/10.1016/j.cardiores.2005.04.032

  18. Bonsignore MR, Morici G, Riccioni R, Huertas A, Petrucci E, Veca M, et al. Hemopoietic and angiogenic progenitors in healthy athletes: different responses to endurance and maximal exercise. J Appl Physiol (1985). 2010;109(1):60–7. http://dx.doi.org/10.1152/japplphysiol.01344.2009

Published
2016-04-15
How to Cite
1.
Santosa M, Ilyas EI, Antarianto RD. The effect of moderate-intensity acute aerobic exercise duration on the percentage of circulating CD31<sup>+</sup&gt; cells in lymphocyte population. Med J Indones [Internet]. 2016Apr.15 [cited 2024Oct.5];25(1):51-6. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1277
Section
Clinical Research