Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration

  • Robert Stefanus Biomedical Program, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Sophie Yolanda Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Radiana D. Antarianto Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
Keywords: aerobic exercise, astrocytes reactivity, GFAP, HSP27, synaptic plasticity
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Background: Glial fibrillary acidic protein (GFAP) and heat shock protein -27 (HSP27) plasma can be used as the parameters of exercise-induced astrocyte reactivity. The American College of Sports Medicine (ACSM) recommends an exercise of 30 minutes or 10 minutes duration (each performing bout accumulated toward 30 minutes). The aim of this study was to compare GFAP and HSP27 plasma concentrations in young adults undergoing acute moderate-intensity aerobic exercise of different durations (10 minutes vs 30 minutes).

Methods: An experimental study with pre-post design was conducted on 22 participants assigned to either 10 minutes or 30 minutes duration of single bout exercise. Blood sampling was performed before and after the exercise. GFAP and HSP27 plasma levels were measured with ELISA methods. Plasma GFAP and HSP27 levels before and after exercise were analyzed using paired t -test, while GFAP and HSP27 levels after exercise between the two groups were processed using unpaired t-test.

Results: Plasma GFAP concentration decreased significantly (0,45 ng/mL) after 30 minutes of aerobic exercise (p<0.05). Plasma HSP27 concentration decreased significantly (1,71 ng/mL) after 10 minutes of aerobic exercise (p<0.05). No significant difference in plasma GFAP and HSP27 concentrations between 10 minutes (GFAP=0.49 ng/mL; HSP27=2.09 ng/mL) and 30 minutes duration of exercise (GFAP=0.45 ng/mL; HSP27=1,71 ng/mL).

Conclusion: Acute moderate-intensity aerobic exercise with 10- and 30-minutes duration reduces the reactivity of astrocytes indication the increase of the synapse plasticity. The decrease in GFAP concentration occurred after 30 minutes of exercise and the decrease in HSP27 occurred after 10 minutes of exercise. These results showed that the body responds differently to different treatment duration in order to obtain the same effect on the body.


  1. World Health Organization, Geneva. Dementia: a public health priority. Available from: [cited 4 July 2014].

  2. Bernardi C, Tramontina AC, Nardin P, Biasibetti R, Costa AP, Vizueti AF, et al. Treadmill exercise induces hippocampal astroglial alteration in rats. Neural Plast. 2013: 2013:1-10.

  3. Cotman CW, Berchtold NC, Christie LA. Exercise builds brain health: key roles of growth factor cascades and inflammation. Trends Neurosci. 2007;30(9):464-70.

  4. American College of Sports Medicine. ACSM's Guidelines for Exercise Testing and Prescription. 8th ed. Philadelphia: Lippincot Williams & Wilkins; 2010. 380p.

  5. Middeldorp J, Hol EM. GFAP in health and disease. Prog Neurobiol. 2011;93:421-43.

  6. Barr CS, Dokas LA. Glucocorticoids regulate the synthesis of HSP27 in rat brain slices. Brain Res. 1999;847:9-17.

  7. Periard JD, Ruell P, Caillaud C, Thompson MW. Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity. Cell Stress Chaperon. 2012;17:375-83.

  8. Perng MD, Cairns L, Van Den Ijssel P, Prescott A, Hutcheson AM, Quinlan RA. Intermediate filament interactions can be altered by HSP27 and aB-crystallin. J Cell Sci. 1999;112:2099-112.

  9. Mayer CA, Brunkhorst R, Niessner M, Pfeilschifter W, Steinmetz H, Foerch C. Blood levels of glial fibrillary acidic protein (GFAP) in patients with neurological diseases. Plos One. 2013;8(4):1-4.

  10. Vissers JLM, Mersch MEC, Rosmalen CF, Van Heumen MJ, Van Geel WJ, Lamers KJ et al. Rapid immunoassay for the determination of glial fibrillary acidic protein (GFAP) serum. Clin Chim Acta. 2006;366:336-40.

  11. Departemen Kesehatan Republik Indonesia Direktorat Jenderal Bina Kesehatan Masyarakat. Petunjuk teknis pengukuran kebugaran jasmani. Jakarta: Departemen Kesehatan RI; 2005. 62p.

  12. De Kloet ER, Joels M, Holsboer F. Stress and the brain: from adaptation to disease. Nat Rev Neurosci. 2005;6:463-75.

  13. Hill EE, Zack E, Battaglini C, Viru M, Viru A, Hackney AC. Exercise and circulating cortisol levels: the intensity threshold effect. J. Endocrinol. 2008;31:587-91.

  14. Nichols NR, Agolley D, Zieba M, Bye N. Glucocorticoid regulation of glial responses during hippocampal neurodegeneration and regeneration. Brain Res Rev. 2005;48:287-301.

  15. Woiciechowsky C, Schoning B, Stoltenburg-Didinger G, Stockhammer F, Volk HD. Brain IL-1β triggers astrogliosis through induction of IL-6: inhibition by propanolol and IL-10. Med Sci Monit. 2004;10(9):325-30.

  16. Jack CS, Arbour N, Manusow J, Montgrain V, Blain M, McCrea E, et al. TLR signaling tailors innate immune responses in human microglia and astrocytes. J Immunol. 2005; 175:4320-30.

  17. Ferns G, Shams S, Shafi S. Heat shock protein 27: its potential role in vascular disease. Int J Exp Pathol. 2006; 86:253-74.

  18. Satoh J, Kim Su. Cytokines and growth factors induce HSP27 phosphorylation in human astrocytes. J Neuropathol Exp Neurol. 1995;54(4):504-12.

  19. Garrido C, Paul C, Seigneuric R, Kampinga HH. The small heat shock proteins family: the long forgotten chaperones. Int J Biochem Cell B. 2012;44:1588-92.

  20. Tabardel Y , Duchateau J, Schmartz D, Marécaux G, Shahla M, Barvais L, et al. Corticosteroids increase blood interleukin-10 levels during cardiopulmonary bypass in men [abstract]. Surgery. 1996;119(1):76-80.

  21. Calderwood SK, Mambula SS, Gray PJ Jr, Theriault JR. Extracellular heat shock protein in cell signaling. FEBS Lett. 2007;581:3689-94.

  22. Flynn MG, McFarlin BK. Toll-like receptor 4: link to the anti-inflammatory effects of exercise. Exerc Sport Sci Rev. 2006;34(4):176-81.

How to Cite
Stefanus R, Yolanda S, Antarianto RD. Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration. Med J Indones [Internet]. 2016Jul.26 [cited 2024May22];25(2):112-7. Available from:
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