Prevention of insulin resistance with <em>Hibiscus sabdariffa</em> Linn. extract in high-fructose fed rat

  • Trinovita Andraini Department of Physiology Faculty of Medicine Universitas Indonesia, Jakarta
  • Sophie Yolanda Department of Physiology Faculty of Medicine, Universitas Indonesia, Jakarta
Keywords: Hibiscus sabdariffa Linn., high-fructose diet, HOMA-IR, insulin resistance
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Abstract

Background: Dyslipidemia and stress oxidative play an important role as the cause of insulin resistance. One herb that has potent antioxidant effect and may improve dyslipidemia is Hibiscus sabdariffa Linn. The aim of this study was to evaluate the effect of Hibiscus sabdariffa Linn. extract on fasting blood glucose level, fasting blood insulin level, and insulin resistance index (HOMA-IR) in high-fructose fed rat.

Methods: This was an experimental study in 25 Sprague-Dawley rats which were administered with a high-fructose diet (10% ad libitum) and Hibiscus sabdariffa Linn. extract at a dose of 100, 200, and 400 mg/kgBW/d simultaneously for 5 weeks. At the end of study, fasting blood glucose level, fasting blood insulin level and insulin resistance index (HOMA-IR) were measured.

Results: Fasting blood glucose, blood insulin, and HOMA-IR level of rats given high-fructose diet with Hibiscus sabdariffa Linn. at dose 100 mg/kgBW/d were not significantly different than the group of rats given only high-fructose fed. While at the dose of 400 mg/kgBW/d, they were significantly lower than the group given only high-fructose fed (4.84 mmol/L vs 6.11 mmol/L, 0.07 µU/L vs 0.3 µU/L, and 0.02 vs 0.08 respectively).

Conclusion: Oral administration of Hibiscus sabdariffa Linn. could prevent the development of insulin resistance induced by high-fructose diet in the rat.


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References

  1. Basciano H, Federico L, Adeli K. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr Metab (Lond). 2005;2(1):5. http://dx.doi.org/10.1186/1743-7075-2-5

  2. Antuna-Puente B, Disse E, Rabasa-Lhoret R, Laville M, Capeau J, Bastard JP. How can we measure insulin sensitivity/resistance? Diabetes Metab. 2011;37(3):179-88. http://dx.doi.org/10.1016/j.diabet.2011.01.002

  3. Altaş M, Var A, Köse C, Özbilgin K, Arı Z. Endothelial dysfunction in high fructose containing diet fed rats: increased nitric oxide and decreased endothelin-1 levels in liver tissue. Dicle Med J Cilt. 2010;37(3):193-8.

  4. Gurrola-Díaz CM, García-López PM, Sánchez-Enríquez S, Troyo-Sanromán R, Andrade-González I, Gómez-Leyva JF. Effects of Hibiscus sabdariffa extract powder and preventive treatment (diet) on the lipid profiles of patients with metabolic syndrome (MeSy). Phytomedicine. 2010;17(7):500-5. http://dx.doi.org/10.1016/j.phymed.2009.10.014

  5. Henriksen EJ, Diamond-Stanic MK, Marchionne EM. Oxidative stress and the etiology of insulin resistance and type 2 diabetes. Free Radic Biol Med. 2011;51(5):993-9. http://dx.doi.org/10.1016/j.freeradbiomed.2010.12.005

  6. Tiganis T. Reactive oxygen species and insulin resistance: the good, the bad and the ugly. Trends Pharmacol Sci. 2011;32(2):82-9. http://dx.doi.org/10.1016/j.tips.2010.11.006

  7. Samuel VT. Fructose induced lipogenesis: from sugar to fat to insulin resistance. Trends Endocrinol Metab. 2011;22(2):60-5. http://dx.doi.org/10.1016/j.tem.2010.10.003

  8. Tee PL, Yusof S, Mohamed S, Umar NA, Mustapha NM. Effect of roselle (Hibiscus sabdariffa L.) on serum lipids of Sprague Dawley rats. Nutr Food Sci. 2002;32(5):190-6. http://dx.doi.org/10.1108/00346650210445758

  9. Hou DX, Fujii M, Terahara N, Yoshimoto M. Molecular mechanisms behind the chemopreventive effects of anthocyanidins. J Biomed Biotechnol. 2004;2004(5):321-5. http://dx.doi.org/10.1155/S1110724304403040

  10. Mahadevan N, Shivali, Kamboj P. Hibiscus sabdariffa Linn – An overview. Nat Prod Rad. 2008;8(1):77-83.

  11. Tsai PJ, McIntosh J, Pearce P, Camden B, Jordan BR. Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L.) extract. Food Research Int. 2002;35(4):351-6. http://dx.doi.org/10.1016/S0963-9969(01)00129-6

  12. Hopkins AL, Lamm MG, Funk JL, Ritenbaugh C. Hibiscus sabdariffa L. in the treatment of hypertension and hyperlipidemia: A comprehensive review of animal and human studies. Fitoterapia. 2013;85:84-94. http://dx.doi.org/10.1016/j.fitote.2013.01.003

  13. Lila MA. Anthocyanins and human health: an in vitro investigative approach. J Biomed Biotechnol. 2004;2004(5):306-13. http://dx.doi.org/10.1155/S111072430440401X

  14. Bunbupha S, Pakdeechote P, Kukongviriyapan U, Pannangpetch P. Antihyperinsulinemia effect of Hibiscus sabdariffa extract in high fructose diet induced insulin resistance rats. Srinagarind Med J. 2012;27:140-3.

  15. Abdulla MH, Sattar MA, Johns EJ. The relation between fructose-induced metabolic syndrome and altered renal haemodynamic and excretory function in the rat. Int J Nephrology. 2011;2011:934659. http://dx.doi.org/10.4061/2011/934659

  16. Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats. Plant food Hum Nutr. 2007;62(1):1-6. http://dx.doi.org/10.1007/s11130-006-0031-7

  17. Suwannaphet W, Meeprom A, Yibchok-Anun S, Adisakwattana S. Preventive effect of grape seed extract against high-fructose diet-induced insulin resistance and oxidative stress in rats. Food Chem Toxicol. 2010;48(7):1853-7. http://dx.doi.org/10.1016/j.fct.2010.04.021

  18. Mardiah, Zakaria FR, Prangdimurti E, Damanik R. The effect of Roselle extract (Hibiscus sabdariffa Linn.) on blood glucose level and total antioxidant level on diabetic rat induced by streptozotocin. IOSR J Pharmacy.2014;4(10):8-16. http://dx.doi.org/10.9790/3013-0401008016

  19. Peng CH, Chyau CC, Chan KC, Chan TC,Wang CJ, Huang CN. Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative-stress while improving insulin resistance. J Agric Food Chem. 2011;59(18):9901-9. http://dx.doi.org/10.1021/jf2022379

Published
2015-01-27
How to Cite
1.
Andraini T, Yolanda S. Prevention of insulin resistance with <em>Hibiscus sabdariffa</em&gt; Linn. extract in high-fructose fed rat. Med J Indones [Internet]. 2015Jan.27 [cited 2019Jul.24];23(4):192-6. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/848
Section
Basic Medical Research