Microbiology aspect of wound infection: in-vitro test for efficacy of hydrophobic dressing in microorganism binding
Aim To do in vitro test to assess the efficacy of hydrophobic dressing CutimedÂ® SorbactÂ® to bind multiresistant bacteriaÂ that caused wound infection, the methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa.
Method This was a cross sectional study that was conducted in the Department of Microbiology, Faculty of Medicine,Â University of Indonesia, on January 2009. In-vitro testing of sterile hydrophobic dressing to bind microorganisms wasÂ conducted by counting MRSA and Pseudomonas aeruginosa that were bound to 1 square centimetre of single layer
sterile hydrophobic dressing (CutimedÂ® SorbactÂ®). Every test was done in triplicate at 0.5, 1, 5, 10, 30 minutes, 1,Â 2, 3, and 4 hours. To compare the hydrophobic dressing capability to bind microorganisms, in vitro testing of sterileÂ conventional dressing to bind microorganisms on 0.5 minutes and 2 hours was done.
Result The binding capacity of sterile hydrophobic dressing began at 0.5 minutes and teached a maximum at 2Â hours. Compared with conventional dressing, sterile hydrophobic dressing had more binding capability to MRSA andÂ Pseudomonas aeruginosa.
Conclusion Hydrophobic dressing (CutimedÂ® SorbactÂ®) had a higher capability to bind MRSA and PseudomonasÂ aeruginosa compared to conventional dressing. (Med J Indones 2009;18:155-60)
Key words: hydrophobicity, MRSA, Pseudomonas aeruginosa
Copyright (c) 2009 Yeva Rosana, Beti E. Dewi, Conny R. Tjampakasari
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