Correlation between oxidative stress and tumor grade in glioma cells from patients in Jakarta

  • Novi S. Hardiany
  • Wawan Mulyawan
  • Septelia I. Wanandi
Keywords: Glioma, Oxidative stress, oxidative stress
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Background: The goal of this study was to analyze the correlation of oxidative stress in human glioma cells with tumor grade in order to explore the role of oxidative stress as a marker in determining the tumor progression.

Methods: Samples were 21 brain tumors and 5 normal brain tissues from glioma patients. Oxidative stress was analyzed by measuring malondialdehyde (MDA), carbonyl and 8-hydroxy-2’-deoxyguanosine (8-OhdG). Additionaly, we analyzed MnSOD expression by measuring the MnSOD mRNA using real time RT-PCR and MnSOD enzyme activity using RanSOD kit. Tumor grade was determined by histopathologic examination. Data was statistically analyzed using t-test and Pearson correlation.

Results: Levels of MDA, carbonyl and 8-OHdG reflecting oxidative stress in glioma cells were significantly higher than in normal brain tissue. The MDA and carbonyl levels were significantly correlated with tumor grade. Relative expression of MnSOD mRNA and specific enzyme activity in glioma cells were significantly higher than in normal brain cells. The relative expression of MnSOD mRNA increased significantly in accordance with the tumor grade. Surprisingly, MnSOD specific activity was significantly lower in high grade than in low grade glioma indicating a discrepancy between mRNA synthesis and its enzyme specific activity. Furthermore, there was a positive correlation between MnSOD mRNA and MDA levels.

Conclusion: The high level of oxidative damage in human glioma cells was significantly correlated with tumor grade. The high level of MnSOD expression in human glioma cells was correlated with the high level of oxidative damage. (Med J Indones. 2012;21:122-7)


  1. Halliwell B, Gutteridge JMC. Free radical in biology and medicine. 4th ed. London: Oxford University Press; 2007. p.30-74.

  2. Monteiro hP, Stern A. Redox modulation of tyrosine phosphorylation-dependent signal transduction pathways. Free Radic Biol Med. 1996;21:323-33.

  3. Halliwell B. Oxidative stress and cancer: have we moved forward?. Biochem J. 2007; 401; 1-11.

  4. Hardiany NS, Sadikin M, Wanandi SI. Gene expression of manganese superoxide dismutase (MnSOD) in human glioma cells. Med J Indones. 2010;19(1):21-5.

  5. Ganesamoni R, Bhattacharyya S, Kumar S, Chauhan A, Mete UK, Agarwal MM et al. Status of oxidative stress in patients with renal cell carcinoma. J Urology. 2011; 187(4): 1172-6.

  6. Bancel B, Esteve J, Souquet JC, Toyokuni S, Ohshima h, Pignatelli B. Differences in oxidative stress dependence between gastric adenocarcinoma subtypes. World J Gastroenterol. 2006; 12(7): 1005-12.

  7. Brown NS, Bicknell R. Hypoxia and oxidative stress in breast cancer. Oxidative stress: its effects on the growth, metastatic potential and response to therapy of breast cancer. Breast Cancer Res. 2001; 3: 323-7.

  8. Aman RA. Identifikasi faktor prediksi radiosensitivitas tumor sel glia: tinjauan khusus pada angiogenesis, proliferasi sel dan apoptosis sebagai perangai biologik tumor. [Disertasi]. Jakarta: Fakultas Kedokteran Universitas Indonesia; 2008.

  9. Wills ED. Evaluation of lipid peroxidation in lipids and biological membranes. In: Snell K and Mullock B editors. Biochemical Toxicology. Washington, DC: IRL Press, 1987; p. 127-52.

  10. Amici A, Levine RL, Tsai L, Stadtman ER. Conversion of amino acid residues in proteins and amino acids homopolymers to carbonyl derivatives by metal-catalyzed

  11. oxidation reactions. J Biol Chem. 1989:264; 3341-6.

  12. Cobbs CS, Levi DS, Aldape K, Israel MA. Manganese superoxide dismutase expression in human central nervous system tumours. Cancer Res. 1996;56:3192-5.

  13. Gonenc A, Ozkan Y, Torun M, Simsek B. Plasma malondialdehyde (MDA) levels in breast and lung cancer patients. J Clin Pharm Ther. 2001;26(2):141-2.

  14. Yilmaz N, Dulger h, Kiymaz N, Yilmaz C, Bayram I, Ragip B, et al. Lipid peroxidation in patients with brain tumor. Int J Neurosci. 2006;116(8):937-43.

  15. Sinha RJ, Singh R, Mehrotra S, Singh SK. Implication of free radical and antioxidant levels in carcinoma of the breast: a never-ending battle for survival. Indian J Cancer. 2009;46(2):146-50.

  16. Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta. 2003;329:23-38

How to Cite
Hardiany NS, Mulyawan W, Wanandi SI. Correlation between oxidative stress and tumor grade in glioma cells from patients in Jakarta. Med J Indones [Internet]. 2012Aug.1 [cited 2022Jul.4];21(3):122-7. Available from:
Basic Medical Research

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