Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity

  • Nani Dharmasetiawani
  • Herman Suryadi
  • Agus Firmansyah
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Abstract

Determination of intestinal lactase activity is directly done by measuring its activity in intestinal epithelium. This is an invasive method and ethically can not be done in healthy infants. Indirectly, determination of lactase activity, stated as excretion and ingestion ratio of lactose and lactulose, needs 30 hours hospitalized infants. The aim of this study was to look for a method for determination of lactase activity which is not invasive and not necessary hospitalized. Using this method  lactose and lactulose were given as a single oral load after 2 hours fasting. Urine were collected for 5 hours starting from consuming sugar solution and then lactose and lactulose concentration in the urine were measured by High Performance Liquid Chromatography. The results showed single oral load of lactose and lactulose can be used for determination of lactase activity in infant and the infants were observed only for 7 hours. (Med J Indones 2003; 12: 8-12) 

Keywords: Lactase activity, Lactulose, Lactose, High Performance Liquid Chromatography

Author Biographies

Nani Dharmasetiawani
Department of Child Health, Budi Kemuliaan Maternity
Hospital, Jakarta, Indonesia
Herman Suryadi
Department of Pharmacy, Faculty of Natural Science,
University of Indonesia, Jakarta, Indonesia
Agus Firmansyah
Department of Child Health, Medical Faculty, University of
Indonesia, Jakarta, Indonesia
Published
2003-02-01
How to Cite
1.
Dharmasetiawani N, Suryadi H, Firmansyah A. Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity. Med J Indones [Internet]. 2003Feb.1 [cited 2020Aug.14];12(1):8-12. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/84
Section
Basic Medical Research

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