The effect of Bifidobacterium animalis lactis HNO19 supplementation among pregnant and lactating women on interleukin-8 level in breast milk and infant’s gut mucosal integrity

  • Naomi E.F. Dewanto Faculty of Medicine, Universitas Indonesia, Jakarta
  • Agus Firmansyah Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Ali Sungkar Department of Obstetric Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Nani Dharmasetiawani Department of Pediatric, Budi Kemuliaan Hospital, Jakarta
  • Sudigdo Sastroasmoro Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Siti B. Kresno Department of Pathology Clinic, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Rulina Suradi Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Saptawati Bardosono Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Dwi Prasetyo Department of Child Health, Faculty of Medicine, Universitas Padjadjaran, Sumedang
Keywords: Bifidobacterium lactis animalis HNO19, breastmilk, gut mucosal integrity
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Abstract

Background: Newborn’s gut mucosal is not fully developed, therefore infants are prone to diarrhea. Probiotic supplementation is known to induce the gut mucosal maturity. This study aimed to identify whether probiotics supplementation among pregnant women since the third trimester would increase the infant’s gut mucosal integrity.

Methods: A double-blind, randomized clinical trial was conducted to understand the potential effect of probiotic supplementation on the level of probiotics and IL-8 in breastmilk, urine IFABP, faecal α-1-antytripsin (AAT) and calprotectin in infant’s at birth (V0) and three-months old (V3). A single strain of Bifidobacterium lactis animalis HNO19 (known as DR10) was used since it was not the resident bacteria. The study was held at Budi Kemuliaan Hospital and its satellite clinics from December 2014 to December 2015.

Results: About 14% (5/35) and 20% (7/35) of the subjects had DR10 in the breastmilk’s colostrum and at the age of 3-months. The median values of IL-8 in the probiotic group vs the placebo group at V0 and V3 were 2810,1 pg/mL vs 1516.4 pg/mL (p=0.327) and 173.2 pg/mL vs 132.7 pg/mL (p=0.211) respectively. IFABP level 211.7 ng/mL vs 842.5 ng/mL (p=0.243) and 25.3 ng/mL vs 25.1 ng/mL (p=0.466); AAT 136.2 mg/dL vs 148.1 mg/dL (p=0.466) and 24 mg/mL vs 29.72 mg/mL (p=0.545); Calprotectin 746.8 ng/mL vs 4645.2 ng/mL (p=0.233) and 378.6 ng/mL vs 391.3 ng/mL (p=0.888).

Conclusion: Probiotic DR10 given to pregnant women since the 3rd trimester can be found in colostrum and 3-months breastmilk. However, it did not affect the level of other probiotics or IL-8 and the gut mucosal integrity.

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Author Biographies

Naomi E.F. Dewanto, Faculty of Medicine, Universitas Indonesia, Jakarta
Pediatric Department
Agus Firmansyah, Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta
Pediatric gastroenterology
Ali Sungkar, Department of Obstetric Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta
Obstetric Gynecology Department
Nani Dharmasetiawani, Department of Pediatric, Budi Kemuliaan Hospital, Jakarta
Pediatric Department
Sudigdo Sastroasmoro, Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta
Pediatric Departement
Siti B. Kresno, Department of Pathology Clinic, Faculty of Medicine, Universitas Indonesia, Jakarta
Pathology Clinic Department
Rulina Suradi, Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta
Pediatric Department
Saptawati Bardosono, Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Jakarta
Nutrition Department
Dwi Prasetyo, Department of Child Health, Faculty of Medicine, Universitas Padjadjaran, Sumedang
Pediatric Department

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Published
2017-11-27
How to Cite
1.
Dewanto NE, Firmansyah A, Sungkar A, Dharmasetiawani N, Sastroasmoro S, Kresno SB, Suradi R, Bardosono S, Prasetyo D. The effect of <em>Bifidobacterium animalis lactis</em&gt; HNO19 supplementation among pregnant and lactating women on interleukin-8 level in breast milk and infant’s gut mucosal integrity. Med J Indones [Internet]. 2017Nov.27 [cited 2019Aug.22];26(3):204-11. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1481
Section
Clinical Research