The effect of moderate-intensity acute aerobic exercise duration on the percentage of circulating CD31<sup>+</sup> cells in lymphocyte population

  • Mariani Santosa Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Ermita I.I. Ilyas Department of Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Radiana D. Antarianto Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
Keywords: CD31 peripheral blood mononuclear cells, circulating endothelial progenitor cells, endothelial regeneration, exercise

Abstract

Background: The increasing number of circulating CD31+ endothelial progenitor cells is one of the important factors for maintaining vascular homeostasis. Exercise will effectively increase the number of circulating CD31+ endothelial progenitor cells. This study aims to determine the effect of moderate-intensity acute aerobic exercise duration on the percentage of circulating CD31+ cells in untrained healthy young adult subjects.

Methods: This study was an experimental study. Untrained healthy volunteers (n=20) performed ergocycle at moderate-intensity (64–74% maximum heart rate) for 10 minutes or 30 minutes. Immediately before and 10 minutes after exercise, venous blood samples were drawn. The percentage of CD31+ cells in peripheral blood was analyzed using flow cytometry. Data was statistically analyzed using student t-test.

Results: There were no significant differences in the mean percentage of circulating CD31+ cells before and after exercise for 10 minutes and 30 minutes (p>0.05). However, there was a different trend in the percentage of circulating CD31+ cells after exercise for 10 minutes and 30 minutes. In the 10 minutes duration, 50% of subjects showed increase. Whereas in the 30 minutes duration, 80% of subjects showed increase.

Conclusion: The percentage of circulating CD31+ cells before and after exercise for 10 minutes was not different compared to 30 minutes. However, data analysis shows that majority of subjects (80%) had increased in the percentage of circulating CD31+ cells after 30 minutes exercise.

References

Institute of Medicine (US) Committee on Preventing the Global Epidemic of Cardiovascular Disease. Promoting cardiovascular health in the developing world: a critical challenge to achive global health. Fuster V, Kelly BB, editors. Washington (DC): National Academies Press (US); 2010. p. 49.

World Health Organization. Preventing chronic disease: a vital investment. Geneva: World Health Organization; 2005. p. 15.

Rajendran P, Rengarajan T, Thangavel J, Nishigaki Y, Sakthisekaran D, Sethi G, et al. The vascular endothelium and human diseases. Int J Biol Sci. 2013;9(10):1057–69. http://dx.doi.org/10.7150/ijbs.7502

Deanfield JE, Halcox JP, Rabelink TJ. Endothelial function and dysfunction: testing and clinical relevance. Circulation. 2007;115(10):1285–95. http://dx.doi.org/10.1161/CIRCULATIONAHA.106.652859

De Biase C, De Rosa R, Luciano R, De Luca S, Capuano E, Trimarco B, et al. Effects of physical activity on endothelial progenitor cells (EPCs). Front Physiol. 2013;4:414. http://dx.doi.org/10.3389/fphys.2013.00414

Timmermans F, Plum J, Yöder MC, Ingram DA, Vandekerckhove B, Case J. Endothelial progenitor cells: identity defined?. J Cell Mol Med. 2009;13(1):87–102. http://dx.doi.org/10.1111/j.1582-4934.2008.00598.x

Kim H, Cho HJ, Kim SW, Liu B, Choi YJ, Lee J, et al. CD31+ cells represent highly angiogenic and vasculogenic cells in bone marrow: novel role of nonendothelial CD31+ cells in neovascularization and their therapeutic effects on ischemic vascular disease. Circ Res. 2010;10(5):602–14. http://dx.doi.org/10.1161/CIRCRESAHA.110.218396

Koutroumpi M, Dimopoulos S, Psarra K, Kyprianou T, Nanas S. Circulating endothelial and progenitor cells: evidence from acute and long term exercise effects. World J Cardiol. 2012;4(12):312–26. http://dx.doi.org/10.4330/wjc.v4.i12.312

Siddique A, Shantsila E, Lip GYH, Varma C. Endothelial progenitor cells: what use for the cardiologist?. J Angiogenes Res. 2010;2(6):1–13. http://dx.doi.org/10.1186/2040-2384-2-6

American College of Sports Medicine. ACSM's guidelines for exercise testing and prescription. 8th ed. Philadelphia: Lippincott Wiliams & Wilkins; 2010. p. 7–76.

Silva JF, Rocha NG, Nóbrega AC. Mobilization of endothelial progenitor cells with exercise in healthy individuals: a systematic review. Arq Bras Cardiol. 2012;98(2):182–91. http://dx.doi.org/10.1161/hc2301.092122

Sharkey BJ. Physiology of fitness. 3rd ed. USA: Human Kinetics Publishers; 1990. p. 19.

Hristov M, Erl W, Weber PC. Endothelial progenitor cells: mobilization, differentiation, and homing. Arterioscler Thromb Vasc Biol. 2003;23(7):1185–9. http://dx.doi.org/10.1161/01.ATV.0000073832.49290.B5

Laufs U, Urhausen A, Werner N, Scharhag J, Heitz A, Kissner G, et al. Running exercise of different duration and intensity: effect on endothelial progenitor cells in healthy subjects. Eur J Cardiovasc Prev Rehabil. 2005;12(4):407–14. http://dx.doi.org/10.1097/01.hjr.0000174823.87269.2e

Cubbon RM, Murgatroyd SR, Ferguson C, Bowen TS, Rakobowchuk M, Baliga V, et al. Human exercise-induced circulating progenitor cell mobilization is nitric oxide-dependent and is blunted in South Asian men. Arterioscler Thromb Vasc Biol. 2010;30(4):878–84. http://dx.doi.org/10.1161/ATVBAHA.109.201012

Swain DP, Abernathy KS, Smith CS, Lee SJ, Bunn SA. Target heart rates for the development of cardiorespiratory fitness. Med Sci Sports Exerc. 1994;26:112–6. http://dx.doi.org/10.1249/00005768-199401000-00019

Kojda G, Hambrecht R. Molecular mechanisms of vascular adaptations to exercise. Physical activity as an effective antioxidant therapy?. Cardiovasc Res. 2005;67(2):187–97. http://dx.doi.org/10.1016/j.cardiores.2005.04.032

Bonsignore MR, Morici G, Riccioni R, Huertas A, Petrucci E, Veca M, et al. Hemopoietic and angiogenic progenitors in healthy athletes: different responses to endurance and maximal exercise. J Appl Physiol (1985). 2010;109(1):60–7. http://dx.doi.org/10.1152/japplphysiol.01344.2009

Published
2016-04-15
How to Cite
1.
Santosa M, Ilyas EI, Antarianto RD. The effect of moderate-intensity acute aerobic exercise duration on the percentage of circulating CD31<sup>+</sup&gt; cells in lymphocyte population. Med J Indones [Internet]. 2016Apr.15 [cited 2024Mar.2];25(1):51-6. Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/1277
Section
Clinical Research