Effects of <em>Hibiscus sabdariffa</em> Linn. on insulin-like growth factor binding protein 3 (IGFBP-3) to prevent overtraining syndrome
DOI:
https://doi.org/10.13181/mji.v23i4.991Keywords:
Hibiscus sabdariffa Linn., IGFBP-3, overtrainingAbstract
Background: Excessive physical exercises (overtraining) can increase the production of reactive oxygen species (ROS). One of the indicators of overtraining syndrome is a decrease in insulin-like growth factor binding protein 3 (IGFBP-3). Administration of Hibiscus sabdariffa Linn., a powerful antioxidant, is expected to boost endogenous antioxidants, and thus prevents overtraining. The aim of this study is to determine the effect of H. sabdariffa on IGFBP-3 levels in rats under "overtraining physical excersice".
Methods: This experimental study was conducted on 30 male rats (Rattus norvegicus 200-250 grams), randomly allocated into 5 groups: 1) control group (C); 2) control with H. sabdariffa (C-Hib); 3) mild aerobic exercise (A-Ex); 4) overtraining exercise (OT); 5) overtraining exercise with H. Sabdariffa (OT-Hib). H. sabdariffa (400 mg/kg/d, 11 weeks) were administered orally via syringe cannula. IGFBP-3 was measured by using ELISA (Cusa bio kit) and data were analyzed with ANOVA test.
Results: Plasma level of IGFBP-3 in the C and OT groups were 17.4 ± 10 mIU/L, the lowest in OT groups (10.7 ± 9.9 mIU/L) and the OT-Hib group had the highest level (31.5 ± 6.2 mIU/L). There was significant difference of the level IGFBP-3 in OT groups with A-Ex groups (10.7 ± 9.9 vs 23.5 ± 9.7 mIU/L; p < 0,05). The significant difference was also observed in the level of IGFBP 3 between C groups and the OT-Hib groups (17.4 ± 10 vs 31.5 ± 6.2; p < 0.05).
Conclusion: Administration of H. sabdariffa can prevent the decrease of IGFBP-3 levels in overtraining rats, indicating its role in preventing overtraining syndrome.
Downloads
References
Bischler TK. The utility of resting levels of IGF-I and IGFBP-3 as markers of training status in elite athletes [thesis]. Canada: University of Lethbridge; 2007 [cited 2012 Jun 6]. Available from:https://www.uleth.ca/dspace/handle/10133/651
Cunha GS, Ribeiro JL, Oliveira AR. Overtraining: theories, diagnosis and markers. Rev Bras Med Esporte. 2006;12(5):267e-71e.
Kreher JB, Schwartz JB. Overtraining syndrome: a practical guide. Sports Health. 2012;4(2):128-38. http://dx.doi.org/10.1177/1941738111434406
Brenner JS; American Academy of Pediatrics Council on Sports Medicine and Fitness. Overuse injuries, overtraining, and burnout in child and adolescent athletes. Pediatrics. 2007;119(6):1242-5. http://dx.doi.org/10.1542/peds.2007-0887
Fry AC, Schilling BK, Weiss LW, Chiu LZ. Z. v2-Adrenergic receptor downregulation and performance decrements during high-intensity resistance exercise overtraining. J Appl Physiol (1985). 2006;101(6):1664-72. http://dx.doi.org/10.1152/japplphysiol.01599.2005
Marcello BM. Overtraining in sport: physiological, psychological and performance effect of participation in division I competitive softball [dissertation]. Texas: Baylor University; 2006 [cited 2012 jun 17]. Available from: https://beardocs.baylor.edu/xmlui/handle/2104/4959
Elloumi M, El Elj N, Zaouali M, Maso F, Filaire E, Tabka Z, et al. IGFBP-3, a sensitive marker of physical training and overtraining. Br J Sports Med. 2005;39(9):604-10. http://dx.doi.org/10.1136/bjsm.2004.014183
Capoluongo E, Pitocco D, Santonocito C, Concolino P, Santini SA, Manto A, et al. Association between serum free IGF-I and IGFBP-3 levels in type-I diabetes patients affected with associated autoimmune disease or diabetic complications. Eur Cytokine Netw. 2006;17(3):167- 74.
Wang SC, Lee SF, Wang CJ, Lee CH, Lee WC, Lee HJ. Aqueous Extract from Hibiscus sabdariffa Linnaeus Ameliorate Diabetic Nephropathy via Regulating Oxidative Status and Akt/Bad/14-3--3g in an Experimental Animal Model. Evid Based Complement Alternat Med. 2011;2011:938126. http://dx.doi.org/10.1093/ecam/nep181
Hohl R, Ferraresso RL, De Oliveira RB, Lucco R, Brenzikofer R, De Macedo DV. Development and characterization of an overtraining animal model. Med Sci Sports Exerc. 2009 May;41(5):1155-63. http://dx.doi.org/10.1249/MSS.0b013e318191259c
Mahadevan N, Shivali, Kamboj P. Hibiscus Sabdariffa Linn.- An overview. NPR. 2008;8(1):77-83.
Kowalczyk E, Krzesi?ski P, Kura M, Szmigiel B, B?aszczyk J. Anthocyanins in medicine. Pol J Pharmacol. 2003;55(5):699-702.
Margonis K, Fatouros IG, Jamurtas AZ, Nikolaidis MG, Douroudos I, Chatzinikolaou A, et.al. Oxidative stress biomarkers responses to physical overtraining: implications for diagnosis. Free Radic Biol Med. 2007;43(6):901-10. http://dx.doi.org/10.1016/j.freeradbiomed.2007.05.022
Xiao W, Chen P, Dong J. Effect of overtraining on sceletal muscle growth and gene expression. Int J Sports Med. 2012 Oct;33(10):846-53. http://dx.doi.org/10.1055/s-0032-1311585
Rosendal L, Langberg H, Flyvbjerg A, Frystyk J, Ørskov H, Kjaer M. Physical capacity influences the response of insulin -like growth factor and its binding protein to training. J Appl Physiol (1985). 2002;93(5):1669-75.
Goodman HM. Basic Medical Endocrinology. 4th ed. China: Elsevier; 2009.
Yadav S, Khrisnamurthy S. Insulin-like growth factors and growth hormone deficiency. Indian Pediatr. 2007;44(5):349-53.
Taipale RS, Häkkinen K. Acute hormonal and force responses to combined strength and endurance loadings in men and woman: The "Order Effect". PLoS ONE. 2013;8(2):e55051. http://dx.doi.org/10.1371/journal.pone.0055051
Banerjee AK, Mandal A, Chanda D, Chakraborti S. Oxidant, antioxidant and physical exercise. Mol Cell Biochem. 2003;253(1-2):307-12. http://dx.doi.org/10.1023/A:1026032404105
Essa MM, Subramanian P. Hibiscus sabdariffa affects ammonium chloride induced hyperammonemic rats. Evid Based Complement Alternat Med. 2007;4(3):321-8. http://dx.doi.org/10.1093/ecam/nel087
Daniel D, Aduwamai UH, Blessing V, Michael AA. Antioxidan and Antiperoxidative activities of aqueous extract of Hibiscus sabdariffa L. calyx on alcohol-induced hepatic lipid peroxidation in rats. American Journal of Biochemistry. 2013;3(2):62-65.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with Medical Journal of Indonesia agree to the following terms:
- Authors retain copyright and grant Medical Journal of Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial License that allows others to remix, adapt, build upon the work non-commercially with an acknowledgment of the work’s authorship and initial publication in Medical Journal of Indonesia.
- Authors are permitted to copy and redistribute the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Medical Journal of Indonesia.
