Expressions of stemness markers in keloid tissue

Authors

  • Sri S. Ningsih Master of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta
  • Dewi H. Sari Master of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta
  • Radiana D. Antarianto Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Novi S. Hardiany Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Mohamad Sadikin Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Septelia I. Wanandi Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Sri W.A. Jusman Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta

DOI:

https://doi.org/10.13181/mji.v27i3.1920

Keywords:

ALDH1, keloid, Oct-4, stemness

Abstract

Background: Keloid is an abnormal wound healing process that extends beyond the site of injury. Keloid and tumor’s shared similarity of recurrence suggesting a shared underlying mechanism that involves stemness. Octamer-binding transcription factor-4 (Oct-4) and aldehyde dehydrogenase-1 (ALDH1) are stem cell stemness markers. This study aimed to analyze Oct-4 and ALDH1 expressions in keloid tissues.

Methods: Samples were obtained from keloid tissue excisions from three keloid patients and post-circumcision preputial skin from three healthy donors (normal control) in accordance with the local ethical committee regulation. Total RNA was isolated using TriPure Isolation kit (Ameritech), and expressions of Oct4 and ALDH1 mRNA in keloid and preputial skin were determined by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using Livak method.

Results: The qRT-PCR analysis revealed the expressions of Oct4 and ALDH1 in keloid and preputial skin tissues. Keloid tissues exhibited lower expression levels of Oct-4 and ALDH1 than the preputial skin. The difference was statistically insignificant.

Conclusion: Keloid tissues express Oct-4 and ALDH1 as stemness markers, and the stemness characteristics of keloid might be similar to a normal skin.

Downloads

Download data is not yet available.

References

Goder M, Kornhaber R, Bordoni D, Winkler E, Haik J, Tessone A. Cutaneous basal cell carcinoma arising within a keloid scar: a case report. Onco Targets Ther. 2016;9:4793-6. https://doi.org/10.2147/OTT.S113724

Ma X, Chen J, Xu B, Long X, Qin H, Zhao RC, et al. Keloid-derived keratinocytes acquire a fibroblast-like appearance and an enhanced invasive capacity in a hypoxic microenvironment in vitro. Int J Mol Med. 2015;35(5):1246-56. https://doi.org/10.3892/ijmm.2015.2135

Zhang Q, Yidi W, Ann DK, Messadi DV, Tuan T, Kelly AP, et al. Mechanisms of hypoxic regulation of plasminogen activator inhibitor-1 gene expression in keloid fibroblasts. J Invest Dermatol. 2003;121(5):1005-12. https://doi.org/10.1046/j.1523-1747.2003.12564.x

Zeineddine D, Hammoud AA, Mortada M, Boeuf H. The Oct4 protein: more than a magic stemness marker. Am J Stem Cells. 2014;3(2):74-82.

Samardzija C, Michael Q, Jock KF, Nuzhat A. Attributes of Oct4 in stem cell biology: perspectives on cancer stem cells of the ovary. J Ovarian Res. 201-42. https://doi.org/10.1186/1757-2215-5-37

Deng C, Wang B, Zhang Z, Sun G, Zhu J, Wang D, et al. The expressions of notch genes in human keloid-derived mesenchymal-like stem cells. Zhonghua Zheng Xing Wai Ke Za Zhi. 2014;30(3):197-202.

Grant C, Chudakova DA, Itinteang T, Chibnall AM, Brasch HD, Davis PF, et al. Expression of embryonic stem cell markers in keloid-associated lymphoid tissue. J Clin Pathol. 2016;69(7):643-6. https://doi.org/10.1136/jclinpath-2015-203483

Douville J, Beaulieu R, Balicki D. ALDH1 as a functional marker of cancer stem and progenitor cells. Stem Cells Dev. 2009;18(1):17-26. https://doi.org/10.1089/scd.2008.0055

Lohberger B, Beate R, Nicole S, Markus A, Bernadette LA, Sonja MW, et al. Aldehyde dehydrogenase 1, a potential marker for cancer stem cells in human sarcoma. PLoS One. 2012;7(8):1-10. https://doi.org/10.1371/journal.pone.0043664

Hahn JM, Glaser K, McFarland KL, Aronow BJ, Boyce ST, Supp DM. Keloid-derived keratinocytes exhibit an abnormal gene expression profile consistent with a distinct causal role in keloid pathology. Wound Repair Regen. 2013;21(4):530-44. https://doi.org/10.1111/wrr.12060

Battula VL, Evans KW, Hollier BG, Shi Y, Marini FC, Ayyanan A, et al. Epithelial-mesenchymal transition-derived cells exhibit multilineage differentiation potential similar to mesenchymal stem cells. Stem Cells. 2010;28(8):1435-45. https://doi.org/10.1002/stem.467

Samardzija C, Quinn M, Findlay JK, Ahmed N. Attributes of Oct4 in stem cell biology: perspectives on cancer stem cells of the ovary. J Ovarian Res. 2012;5(37):1-12. https://doi.org/10.1186/1757-2215-5-37

Nakahata K, Shuichiro U, Shimpei N, Miyoko K, Masahiro Z, Takaharu O, et al. Aldehyde dehydrogenase 1 (ALDH1) is a potential marker for cancer stem cells in embryonal rhabdomyosarcoma. PLoS One. 2015;10(4):1-16. https://doi.org/10.1371/journal.pone.0125454

Jumper N, Hodgkinson T, Paus R, Bayat A. Site-specific gene expression profiling as a novel strategy for unravelling keloid disease pathobiology. PLoS One. 2017;12(3):1-33. https://doi.org/10.1371/journal.pone.0172955

Yan L, Rui C, Yuan BL, Lian ZW, Bo P, Xiao YL, et al. MiR-21-5p links epithelial-mMesenchymal transition phenotype with stem-like cell signatures via AKT signaling in keloid keratinocytes. Sci Rep. 2016;6:28281. https://doi.org/10.1038/srep28281

Wang DL, Zhu JJ, Deng CL, Wang B, Yu LM. Identification of biological characteristics of human keloid-derived stem cells. Zhonghua Shao Shang Za Zhi. 2011;27(3):210-4.

Rodemann HP, Rennekampff HO. Functional diversity of fibroblast. In: Mueller MM, Fusenig NE editors. Tumor-associated fibroblast and their matrix. New York: Springer; 2014. P.23-36.

Chipev, Simman R, Hatch G, Katz AE, Siegel DM, Simon M. Myofibroblast phenotype and apoptosis in keloid and palmar fibroblasts invitro. Cell Death Differ. 2000;7:166-76. https://doi.org/10.1038/sj.cdd.4400605

Wulandari E, Jusman SW, Moenadjat Y, Jusuf AA, Sadikin M. Expressions of collagen I and III in hypoxic keloid tissue. Kobe J Med Sci. 2016;62(3):E58-69.

Downloads

Published

2018-10-12

How to Cite

1.
Ningsih SS, Sari DH, Antarianto RD, Hardiany NS, Sadikin M, Wanandi SI, Jusman SW. Expressions of stemness markers in keloid tissue. Med J Indones [Internet]. 2018Oct.12 [cited 2024Nov.19];27(3):145-9. Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/1920

Issue

Vol. 27 No. 3 (2018): September

Section

Basic Medical Research

License

Authors who publish with Medical Journal of Indonesia agree to the following terms:

  1. Authors retain copyright and grant Medical Journal of Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial License that allows others to remix, adapt, build upon the work non-commercially with an acknowledgment of the work’s authorship and initial publication in Medical Journal of Indonesia.
  2. Authors are permitted to copy and redistribute the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Medical Journal of Indonesia.
Abstract viewed = 1033 times

Most read articles by the same author(s)

1 2 3 4 > >>