Novel and simple method using cable ties to induce intestinal strangulation in a rat model

  • Agi Satria Putranto Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Fransiscus Dhyanagiri Suyatna Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia https://orcid.org/0000-0002-3639-4043
  • Vivian Soetikno Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Yefta Moenadjat Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia https://orcid.org/0000-0002-1109-2540
Keywords: collagen, fibrosis, intestinal obstruction
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Abstract

BACKGROUND The pathogenesis of intestinal fibrosis after strangulation in abdominal surgery is not fully understood. Developing an experimental and reliable method is needed to overcome this problem. This study aimed to develop an effective method for intestinal strangulation leading to fibrosis, which might induce intestinal obstruction.

METHODS This study was conducted from 2018 to 2019 at the Department of Pharmacology, Faculty of Medicine, Universitas Indonesia. A total of 24 Sprague Dawley rats were used in this study. Artificial intestinal strangulation using cable ties made of durable plastic, which is generally used to ligate objects, was applied, and rats were further classified into control, 6-hour, and 24-hour strangulation groups. At the end of the study, the rats were sacrificed, intestinal tissues were collected, and histomorphological changes were observed using hematoxylin and eosin stain. Moreover, Masson’s trichome staining was used to assess collagen density.

RESULTS Median collagen density score of the 24-hour strangulation group was higher than the control. A significant difference in collagen density was found between the submucosal layer of the 24-hour strangulation group and the control (45.4 [11.4] versus 32.4 [14.0], p<0.001) and between the mucosa of the 6- and 24-hour strangulation groups and the control (26.9 [17] versus 6.46 [4.3], p = 0.01 and 24.9 [8] versus 6.46 [4.3], p = 0.004, respectively).

CONCLUSIONS The simple use of cable ties adequately promotes intestinal strangulation.

References

  1. Ismael H, Ragoza Y, Cox S. Intestinal stenosis of Garré: a rare cause of small bowel obstruction in the virgin abdomen. Int J Surg Case Rep. 2016;25:156-60. https://doi.org/10.1016/j.ijscr.2016.06.005

  2. Barry HC. Fibrous stricture of the small intestine following strangulated hernia. Br J Surg. 1942;30(117):64-9. https://doi.org/10.1002/bjs.18003011709

  3. Vowles KDJ. Intestinal complications of strangulated hernia. Br J Surg. 1959;47(202):189-92. https://doi.org/10.1002/bjs.18004720216

  4. Speca S, Giusti I, Rieder F, Latella G. Cellular and molecular mechanisms of intestinal fibrosis. World J Gastroenterol. 2012;18(28):3635-61. https://doi.org/10.3748/wjg.v18.i28.3635

  5. Rieder F, Brenmoehl J, Leeb S, Schölmerich J, Rogler G. Wound healing and fibrosis in intestinal disease. Gut. 2007;56(1):130-9. https://doi.org/10.1136/gut.2006.090456

  6. Višnjić S, Car A, Kralj R. Stenosis of the small intestine after reduction of strangulated Littre hernia in an infant. Hernia. 2013;17:285-8. https://doi.org/10.1007/s10029-011-0861-6

  7. Weledji EP, Theophile N. Atypical small bowel obstruction following repair of inguinal hernia: a case of intestinal stenosis of Garre. Trop Doct. 2013;43(2):88-91. https://doi.org/10.1177/0049475513491727

  8. Alvarado A. Fibrosis: the sixth element. Clin Res Trials. 2018;4(6):1-10. https://doi.org/10.15761/CRT.1000241

  9. Lee JJ, Rosenberg HF, editors. Eosinophils in health and disease. Amsterdam: Elsevier; 2013. Chapter 12, Eosinophil-mediated tissue remodeling and fibrosis. p. 391-429. https://doi.org/10.1016/B978-0-12-394385-9.00012-2

  10. Fiocchi C, Lund PK. Themes in fibrosis and gastrointestinal inflammation. Am J Physiol Gastrointest Liver Physiol. 2011;300(5):G677-83. https://doi.org/10.1152/ajpgi.00104.2011

  11. Wynn TA, Vannella KM. Macrophages in tissue repair, regeneration, and fibrosis. Immunity. 2016;44(3):450-62. https://doi.org/10.1016/j.immuni.2016.02.015

  12. Charan J, Kantharia ND. How to calculate sample size in animal studies? J Pharmacol Pharmacother. 2013;4(4):303-6. https://doi.org/10.4103/0976-500X.119726

  13. Visha MG, Karunagaran M. A review on wound healing. Int J Clinicopathol Correl. 2019;3:50-9. https://doi.org/10.4103/ijcpc.ijcpc_13_19

  14. Sengupta P. The laboratory rat: relating its age with human's. Int J Prev Med. 2013;4(6):624-30.

  15. Giroux MC, Hélie P, Burns P, Vachon P. Anesthetic and pathological changes following high doses of ketamine and xylazine in Sprague Dawley rats. Exp Anim. 2015;64(3):253-60. https://doi.org/10.1538/expanim.14-0088

  16. Mimura I, Nangaku M, Nishi H, Inagi R, Tanaka T, Fujita T. Cytoglobin, a novel globin, plays an antifibrotic role in the kidney. Am J Physiol Renal Physiol. 2010;299(5):F1120-33. https://doi.org/10.1152/ajprenal.00145.2010

  17. Chiu CJ, McArdle AH, Brown R, Scott HJ, Gurd FN. Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg. 1970;101(4):478-83. https://doi.org/10.1001/archsurg.1970.01340280030009

  18. Park PO, Haglund U, Bulkley GB, Fält K. The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion. Surgery. 1990;107(5):574-80.

  19. Oltean M, Olausson M. The Chiu/Park scale for grading intestinal ischemia-reperfusion: if it ain't broke don't fix it! Intensive Care Med. 2010;36(6):1095; author reply 1096. https://doi.org/10.1007/s00134-010-1811-y

  20. Theiss AL, Fuller CR, Simmons JG, Liu B, Sartor RB, Lund PK. Growth hormone reduces the severity of fibrosis associated with chronic intestinal inflammation. Gastroenterology. 2005;129(1):204-19. https://doi.org/10.1053/j.gastro.2005.05.019

  21. Yuan ML, Yang Z, Li YC, Shi LL, Guo JL, Huang YQ, et al. Comparison of different methods of intestinal obstruction in a rat model. World J Gastroenterol. 2013;19(5):692-705. https://doi.org/10.3748/wjg.v19.i5.692

  22. Rieder F, editors. Fibrostenotic inflammatory bowel disease. Cham: Springer International Publishing; 2018. https://doi.org/10.1007/978-3-319-90578-5

  23. Gerarduzzi C, Di Battista JA. Myofibroblast repair mechanisms post-inflammatory response: a fibrotic perspective. Inflamm Res. 2017;66(6):451-65. https://doi.org/10.1007/s00011-016-1019-x

Published
2022-06-13
How to Cite
1.
Putranto AS, Suyatna FD, Soetikno V, Moenadjat Y. Novel and simple method using cable ties to induce intestinal strangulation in a rat model. Med J Indones [Internet]. 2022Jun.13 [cited 2022Oct.4];31(2):91-5. Available from: https://mji.ui.ac.id/journal/index.php/mji/article/view/5799
Section
Basic Medical Research