Detection of Surra (trypanosomiasis) positivity in humans in an outbreak area of Indonesia

  • Dyah Haryuningtyas Sawitri Indonesian Research Centre for Veterinary Sciences, Bogor, Indonesia
  • April Hari Wardhana Indonesian Research Centre for Veterinary Sciences, Bogor, Indonesia
  • Mohamad Sadikin Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Heri Wibowo Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
DOI: https://doi.org/10.13181/mji.v28i2.1767
Keywords: Trypanosoma evansi, human serum, CATT/T. evansi, FELISA
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Abstract

BACKGROUND Surra is an infection caused by a blood protozoan parasite, Trypanosoma evansi, and transmitted by blood-sucking insects. The parasite generally infects only animals; however, it was reported to infect an Indian cattle farmer in 2004, followed by reports of other human cases. The most severe Surra outbreak in Indonesia occurred in Sumba Island during 2010-2012, resulting in the death of more than 2,000 livestock. This study was conducted to explore the serological status of farmers who have intensive contact with their livestock against T. evansi infection in Southwest Sumba district.

METHODS A total of 24 serum samples were collected from farmers living in the Surra outbreak area. All sera were tested using both card agglutination test for trypanosomiasis/T. evansi (CATT/T. evansi) and field enzyme-linked immunosorbent assay (FELISA).

RESULTS Of the 24 serum samples, 4 (16.7%) samples were seropositive for the antigen T. evansi using both tests. This is the first report of human trypanosomiasis (Surra) in Indonesia. Unfortunately, the clinical manifestations of farmers with positive Surra infection were not reported because all sera samples used in this study were obtained from the Public Health Service with no reports of clinical signs from the respondents.

CONCLUSIONS Farmers living in the Surra outbreak area have a high risk of being infected with T. evansi due to their potential frequent contact with Surra vectors. Therefore, T. evansi infection in humans requires attention as it might have the potential to develop as a new emerging zoonotic disease in Indonesia.

References

  1. Truc P, Büscher P, Cuny G, Gonzatti MI, Jannin J, Joshi P, et al. Atypical human infections by animal trypanosomes. PLoS Negl Trop Dis. 2013;7(9):e2256. https://doi.org/10.1371/journal.pntd.0002256

  2. Powar RM, Shegokar VR, Joshi PP, Dani VS, Tankhiwale NS, Truc P, et al. A Rare case of human trypanosomiasis caused by Trypanosoma evansi. Indian J Med Microbiol. 2006;24(1):72-4. https://doi.org/10.4103/0255-0857.19904

  3. Gutierrez C, Desquesnes M, Touratier L, Büscher P. Trypanosoma evansi: recent outbreaks in Europe. Vet Parasitol. 2010;174(1-2):26-9. https://doi.org/10.1016/j.vetpar.2010.08.012

  4. Desquesnes M, Dargantes A, Lai DH, Lun ZR, Holzmuller P, Jittapalapong S. Trypanosoma evansi and surra: a review and perspectives on transmission, epidemiology and control, impact, and zoonotic aspects. Biomed Res Int. 2013;2013:321237. https://doi.org/10.1155/2013/321237

  5. Joshi PP, Shegokar VR, Powar RM, Herder S, Katti R, Salkar HR, et al. Human trypanosomiasis caused by Trypanosoma evansi in India: the first case report. Am J Trop Med Hyg. 2005;73(3):491-5. https://doi.org/10.4269/ajtmh.2005.73.491

  6. Vanhollebeke B, Truc P, Poelvoorde P, Pays A, Joshi PP, Katti R, et al. Human Trypanosoma evansi Infection linked to a lack of apolipoprotein L-I. N Engl J Med. 2006;355(26):2752-6. https://doi.org/10.1056/NEJMoa063265

  7. Da Silva AS, Duck MR, Fanfa Vda R, Otto MA, Nunes JT, Tonin AA, et al. Trypanocidal activity of human plasma on Trypanosoma evansi in mice. Rev Bras Parasitol Vet. 2012;21(1):55-9. https://doi.org/10.1590/S1984-29612012000100011

  8. Pérez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homblé F, et al. Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes. Science. 2005;309(5733):469-72. https://doi.org/10.1126/science.1114566

  9. Lun ZR, Reid SA, Lai DH, Li FJ. Atypical human trypanosomiasis: a neglected disease or just an unlucky accident? Trends Parasitol. 2009;25(3):107-8. https://doi.org/10.1016/j.pt.2008.12.001

  10. Lai DH, Wang QP, Li Z, Luckins AG, Reid SA, Lun ZR. Investigations into human serum sensitivity expressed by stocks of Trypanosoma brucei evansi. Int J Parasitol. 2010;40(6):705-10. https://doi.org/10.1016/j.ijpara.2009.11.009

  11. Van Vinh Chau N, Buu Chau L, Desquesnes M, Herder S, Phu Huong Lan N, Campbell JI, et al. A clinical and epidemiological investigation of the first reported human infection with the zoonotic parasite Trypanosoma evansi in Southeast Asia. Clin Infect Dis. 2016;62(8):1002-8. https://doi.org/10.1093/cid/ciw052

  12. Gutierrez C, Juste MC, Corbera J, Magnus E, Verloo D, Montoya JA. Camel trypanosomosis in the Canary Islands: assessment of seroprevalence and infection rates using the card agglutination test (CATT/T. evansi) and parasite detection tests. Vet Parasitol. 2000;90(1-2):155-9. https://doi.org/10.1016/S0304-4017(00)00225-9

  13. Singla L Das, Sharma A, Kaur P, Tuli A, Bhat SA, Bal S, et al. Bovine trypanosomosis in Punjab: assesment of seroprevalence by CATT/T. evansi. Int J Adv Res. 2013;1(9):364-71.

  14. Bajyana Songa E, Hamers R. A card agglutination test (CATT) for veterinary use based on an early VAT RoTat 1/2 of Trypanosoma evansi. Ann Soc Belg Med Trop. 1988;68(3):233-40.

  15. Elhaig MM, Youssef AI, El-Gayar AK. Molecular and parasitological detection of Trypanosoma evansi in camels in Ismailia, Egypt. Vet Parasitol. 2013;198(1-2):214-8. https://doi.org/10.1016/j.vetpar.2013.08.003

  16. Mastra IK. Seroprevalensi trypanosomiasis di Pulau Sumbawa, Propinsi Nusa Tenggara Barat. Bul Vet. 2011;XXIII(79):131-8.

  17. Ngaira J, Bett B, Karanja SM, Njagi EN. Evaluation of antigen and antibody rapid detection test for Trypanosoma evansi infection in camels in Kenya. Vet Parasitol. 2003;114(2):131-41. https://doi.org/10.1016/S0304-4017(03)00112-2

  18. Subekti D, Kusumaningtyas E. ELISA dan aglutinasi lateks. J Ilmu Ternak dan Vet. 2011;224-33.

  19. Ditjenak. Pedoman pengendalian dan pemberantasan penyakit Trypanosomiasis (Surra). Direktorat Kesehatan Hewan, Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementrian Pertanian. 2012.

  20. Verloo D, Holland W, My LN, Thanh NG, Tam PT, Goddeeris B, et al. Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam. Vet Parasitol [Internet]. 2000;92(2):87-96. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0304401700002843 https://doi.org/10.1016/S0304-4017(00)00284-3

  21. Wardhana AH, Suhardono, Sawitri D, Subekti D. Karakterisasi molekuler dan derajat patogenitas Trypanosoma evansi isolat lokal Indonesia pada sapi dan kerbau. Laporan Penelitian APBN Balai Besar Penelitian Veteriner Bogor; 2012.

  22. Shegokar VR, Powar RM, Joshi PP, Bhargava A, Dani VS, Katti R, et al. Short report: human trypanosomiasis caused by Trypanosoma evansi in India: preliminary serologic survey of the local population. Am J Trop Med Hyg. 2006;75(5):869-70. https://doi.org/10.4269/ajtmh.2006.75.869

  23. Ekawasti F, Sawitri D, Wardhana A. Perbandingan Metode Penyimpanan Darah Vektor Surra (Lalat Haematophagus) Untuk Analisis Multiplex Polimerase Chain Reaction. Seminar Nasional Peternakan dan Veteriner. 2014. p. 209-17.

  24. Brun R. Human Asian trypanosomiasis, a new threat to human health? Am J Trop Med Hyg. 2005;73(3):484. https://doi.org/10.4269/ajtmh.2005.73.484

  25. Wabale V, Nalage P, Joshi A, Bharadwaj R, Desphande K, Chowdhary A. Human Asian trypanosomiasis due to Trypanosoma evansi: a rare case. J Adv Parasitol. 2015;2(3):65-8. https://doi.org/10.14737/journal.jap/2015/2.3.65.68

  26. Das S. Trypanosomiasis-India (WestBengal) [Internet].[Cited July 2017]. Available from: http://www.promedmail.org/direct.php? id=20050120.0197.

  27. Haridy FM, El-Metwally MT, Khalil HH, Morsy TA. Trypanosoma evansi in dromedary camel: with a case report of zoonosis in greater Cairo, Egypt. J Egypt Soc Parasitol. 2011;41(1):65-76.

Published
2019-08-09
How to Cite
1.
Sawitri DH, Wardhana AH, Sadikin M, Wibowo H. Detection of Surra (trypanosomiasis) positivity in humans in an outbreak area of Indonesia. Med J Indones [Internet]. 2019Aug.9 [cited 2024Apr.25];28(2):196-02. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1767
Issue
Vol. 28 No. 2 (2019): June
Section
Brief Communication

Copyright (c) 2019 Dyah Haryuningtyas Sawitri

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