TMEPAI genome editing in triple negative breast cancer cells

  • Bantari W.K. Wardhani Doctoral Program in Biomedicine, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Meidi U. Puteri Medical Sciences Master Program, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki
  • Yukihide Watanabe Department of Experimental Pathology, Faculty of Medicine, University of Tsukuba, Ibaraki
  • Melva Louisa Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Rianto Setiabudy Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Mitsuyasu Kato Department of Experimental Pathology, Faculty of Medicine, University of Tsukuba, Ibaraki
Keywords: CRISPR/Cas9, gene editing, knock-out cell lines
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Abstract

Background: Clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) is a powerful genome editing technique. It consists of RNA-guided DNA endonuclease Cas9 and single guide RNA (gRNA). By combining their expressions, high efficiency cleavage of the target gene can be achieved, leading to the formation of DNA double-strand break (DSB) at the genomic locus of interest which will be repaired via NHEJ (non-homologous end joining) or HDR (homology-directed repair) and mediate DNA alteration. We aimed to apply the CRISPR/Cas9 technique to knock-out the transmembrane prostate androgen-induced protein (TMEPAI) gene in the triple negative breast cancer cell line.

Methods: Designed gRNA which targets the TMEPAI gene was synthesized, annealed, and cloned into gRNA expression vector. It was co-transfected into the TNBC cell line using polyethylenimine (PEI) together with Cas9-GFP and puromycin resistant gene vector. At 24-hours post-transfection, cells were selected by puromycin for 3 days before they were cloned. Selected knock-out clones were subsequently checked on their protein levels by western blotting.

Results: CRISPR/Cas9, a genome engineering technique successfully knocked-out TMEPAI in the Hs578T TNBC cell line. Sequencing shows a frameshift mutation in TMEPAI. Western blot shows the absence of TMEPAI band on Hs578T KO cells.

Conclusion: TMEPAI gene was deleted in the TNBC cell line using the genomic editing technique CRISPR/Cas9. The deletion was confirmed by genome and protein analysis.

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Published
2017-05-16
How to Cite
1.
Wardhani BW, Puteri MU, Watanabe Y, Louisa M, Setiabudy R, Kato M. TMEPAI genome editing in triple negative breast cancer cells. Med J Indones [Internet]. 2017May16 [cited 2019Dec.6];26(1):14-8. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/1871
Section
Basic Medical Research