DNA quality and quantity in adipose tissue: a comparison of the effects of bomb explosion

  • Leonardo Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Ade Firmansyah Sugiharto Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Wresti Indriatmi Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Djaja Surya Atmadja Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Ahmad Yudianto Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Herkutanto Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Wahyu Widodo Indonesian National Police, Batam, Kepulauan Riau, Indonesia
DOI: https://doi.org/10.13181/mji.oa.247206
Keywords: adipose tissue, bombs, DNA typing, human identification
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Abstract

BACKGROUND Adipose tissue is often overlooked in DNA testing due to misconceptions about its DNA content. However, its shock-absorbing qualities may be useful for high-pressure scenarios like bomb blasts. This study aimed to evaluate DNA quality and quantity in adipose tissue affected by blasts compared to that in unaffected tissue.

METHODS 10 adipose tissue samples were taken from regions near and far from the blast, representing the blast-exposed and non-blast-exposed groups. The adipose tissue was stored at a low temperature for 5 days, after which an organic extraction method was applied. The purity of the DNA extract was assessed using a NanoDrop spectrophotometer, and its integrity was evaluated using 0.8% concentration gel electrophoresis at 60 V for 90 min. DNA typing was conducted using the GlobalFiler™ kit, and DNA quantity was determined with the Quantifiler™ Trio DNA Quantification kit.

RESULTS Of 20 DNA extracts from adipose tissue, all samples demonstrated purity, integrity, and complete typing results. Adequate integrity was found in 90% of samples in both groups. A 50% incidence of allele shifting was observed at the D7S820 locus within the blast-exposed group.

CONCLUSIONS DNA from blast-exposed adipose tissue exhibited no significant quality or quantity differences from non-blast-exposed tissue. This suggested adipose tissue’s potential as an alternative DNA source in a bomb explosion.

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Published
2024-02-07
How to Cite
1.
Leonardo, Sugiharto AF, Indriatmi W, Atmadja DS, Yudianto A, Herkutanto, Widodo W. DNA quality and quantity in adipose tissue: a comparison of the effects of bomb explosion. Med J Indones [Internet]. 2024Feb.7 [cited 2024May3];32(4):205-11. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/7206
Issue
Vol. 32 No. 4 (2023): December
Section
Basic Medical Research

Copyright (c) 2024 Leonardo, Ade Firmansyah Sugiharto, Wresti Indriatmi, Djaja Surya Atmadja, Ahmad Yudianto, Herkutanto, Wahyu Widodo

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