Effect of hypoxia-inducible factor-1α induction by CoCl2 on breast cancer cells survival: influence of cytochrome-c and survivin

  • Reni Paramita Doctoral student in Biomedical Study, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Mohamad Sadikin Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Noorwati Sutandyo Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Septelia I. Wanandi Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta
Keywords: apoptosis, cell viability, cytochrome-c, hypoxia-inducible factor-1α, survivin
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Abstract

Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.

Methods: Breast cancer cell lines T47D were induced by CoCl2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities.

Results: HIF-1α induction by CoCl2 causes the increase of  protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability.

Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.

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Published
2014-10-15
How to Cite
1.
Paramita R, Sadikin M, Sutandyo N, Wanandi SI. Effect of hypoxia-inducible factor-1α induction by CoCl<sub>2</sub&gt; on breast cancer cells survival: influence of cytochrome-c and survivin. Med J Indones [Internet]. 2014Oct.15 [cited 2019Nov.18];23(3):139-42. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/933
Section
Basic Medical Research