Effect of hypoxia-inducible factor-1α induction by CoCl2 on breast cancer cells survival: influence of cytochrome-c and survivin

Reni Paramita, Mohamad Sadikin, Noorwati Sutandyo, Septelia I. Wanandi



DOI: https://doi.org/10.13181/mji.v23i3.933

Abstract


Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.

Methods: Breast cancer cell lines T47D were induced by CoCl2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities.

Results: HIF-1α induction by CoCl2 causes the increase of  protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability.

Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.


Keywords


apoptosis; cell viability; cytochrome-c; hypoxia-inducible factor-1α; survivin

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