Development of multiplex-PCR assay for rapid detection of Candida spp.

  • Ni Made A. Tarini
  • Mardiastuti H. Wahid
  • Fera Ibrahim
  • Andi Yasmon
  • Samsuridjal Djauzi
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Abstract

Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identification of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp.

Methods Five Candida spp. isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay.

Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.

Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of Candida spp. (Med J Indones 2010; 19:83-7)

Keywords: Candida spp., multiplex-PCR

Published
2010-05-01
How to Cite
1.
Tarini NMA, Wahid MH, Ibrahim F, Yasmon A, Djauzi S. Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em&gt;. Med J Indones [Internet]. 2010May1 [cited 2020Mar.31];19(2):83-7. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/387
Section
Basic Medical Research

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