Simultaneous detection of Legionella species and Legionella pneumophila by duplex PCR (dPCR) assay in cooling tower water samples from Jakarta, Indonesia

  • Andi Yasmon
  • Yusmaniar Yusmaniar
  • Anis Anis
  • Budiman Bela
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Abstract

Aim: Since culture method is time-consuming and has low  sensitivity, we developed a duplex PCR (dPCR) assay for the detection of Legionella sp. and L. pneumophila in cooling tower samples. We used culture method as a gold standard.

Methods: Optimization of dPCR method was performed to obtain an assay with high sensitivity and specifi city. The optimized method was used to detect Legionella sp. dan L. pneumophila in 9 samples obtained from 9 buildings in Jakarta. For culture method, the bacteria were grown or isolated on selective growth factor supplemented-buffered charcoal yeast extract (BCYE) media.

Results: Of 9 samples tested by dPCR assay, 6 were positive for Legionella species,1 was positive for L. pneumophila, and 2 showed negative results. For the same samples, no Legionella sp. was detected by the culture method.

Conclusion: dPCR assay was much more sensitive than the culture method and was potentially used as a rapid, specifi c and sensitive test for routine detection of Legionella sp. dan for L. pneumophila in water samples. (Med J Indones 2010; 19:223-7)

Keywords: BCYE media, mip gene, 16S-rRNA gene

Published
2010-11-01
How to Cite
1.
Yasmon A, Yusmaniar Y, Anis A, Bela B. Simultaneous detection of Legionella species and Legionella pneumophila by duplex PCR (dPCR) assay in cooling tower water samples from Jakarta, Indonesia. Med J Indones [Internet]. 2010Nov.1 [cited 2019Nov.12];19(4):223-7. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/408
Section
Basic Medical Research

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